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| Status |
Public on Sep 17, 2022 |
| Title |
Expression data from Butyroside D treated B16F10 cells [0.2uM] |
| Platform organism |
Mus musculus |
| Sample organism |
Mus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling reveals a potential role of Butyroside D in melanogenesis inhibition in B16F10 cells.
B16F10 cells were murine melanoma cell line, treated with 0.2 μM Butyroside D for 48 h. Microarray gene expression profiling was conducted for two biological replicates
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| Overall design |
RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis using Affymetrix’s GeneChip® System (Affymetrix Inc., Santa Clara, CA, USA) using Mouse Clariom S Array following the manufacturer’s instructions. Complementary RNA (cRNA) was synthesized using IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). The prepared samples were fragmented and labeled and prepared for hybridization using GeneChipTM Hybridization, Wash and Strain Kit. Each specific sample was injected into the GeneChip TM cartridge of Clariom S Array and incubated with rotation of 60 rpm for 16 h at 45℃ in the GeneChip® hybridization oven 645. Arrays were washed and stained with GeneChip® Fluidic station 450 and scanned with GeneChip® Scanner 3000.
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| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 16, 2022 |
| Last update date |
Sep 19, 2022 |
| Contact name |
Meriem Bejaoui |
| E-mail(s) |
mariembejaoui.mb@gmail.com
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| Organization name |
University of Tsukuba
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| Street address |
1 Chome-1-1 Tennodai
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| City |
Tsukuba, |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8577 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE213493 |
Expression data from Butyroside D treated B16F10 cells |
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| Relations |
| BioProject |
PRJNA881224 |
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