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Series GSE213492 Query DataSets for GSE213492
Status Public on Sep 17, 2022
Title Expression data from Butyroside D treated B16F10 cells [2uM]
Platform organism Mus musculus
Sample organism Mus
Experiment type Expression profiling by array
Summary Gene expression profiling reveals a potential role of Butyroside D in melanogenesis inhibition in B16F10 cells.
B16F10 cells were murine melanoma cell line, treated with 2 μM Butyroside D for 48 h. Microarray gene expression profiling was conducted for two biological replicates
 
Overall design RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis using Affymetrix’s GeneChip® System (Affymetrix Inc., Santa Clara, CA, USA) using Mouse Clariom S Array following the manufacturer’s instructions. Complementary RNA (cRNA) was synthesized using IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). The prepared samples were fragmented and labeled and prepared for hybridization using GeneChipTM Hybridization, Wash and Strain Kit. Each specific sample was injected into the GeneChip TM cartridge of Clariom S Array and incubated with rotation of 60 rpm for 16 h at 45℃ in the GeneChip® hybridization oven 645. Arrays were washed and stained with GeneChip® Fluidic station 450 and scanned with GeneChip® Scanner 3000.
 
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Submission date Sep 16, 2022
Last update date Sep 19, 2022
Contact name Meriem Bejaoui
E-mail(s) mariembejaoui.mb@gmail.com
Organization name University of Tsukuba
Street address 1 Chome-1-1 Tennodai
City Tsukuba,
State/province Ibaraki
ZIP/Postal code 305-8577
Country Japan
 
Platforms (1)
GPL23038 [Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay)
Samples (8)
GSM6588069 Control 1
GSM6588070 Control 2
GSM6588071 Control 3
This SubSeries is part of SuperSeries:
GSE213493 Expression data from Butyroside D treated B16F10 cells
Relations
BioProject PRJNA881225

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE213492_RAW.tar 10.0 Mb (http)(custom) TAR (of CEL, CHP)
GSE213492_processed_data.txt.gz 712.8 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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