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| Status |
Public on Oct 10, 2023 |
| Title |
A role for miR-4734 in conditional suppression of ER stress-associated pro-inflammatory responses [miR-CTRL or miR-4734-transfected HFF] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Prolonged metabolic stress can lead to severe pathologies. In metabolically challenged primary fibroblasts, we assigned a novel role for the poorly characterized miR-4734 in restricting ATF4 and IRE1-mediated upregulation of a set of pro-inflammatory cytokines and endoplasmic reticulum (ER) stress-associated genes. Conversely, inhibition of this miRNA augmented the expression of those genes. Mechanistically, miR-4734 was found to restrict the expression of NFKBIZ, that is required for optimal expression of the pro-inflammatory genes and whose mRNA is targeted directly by miR-4734. Concordantly, overexpression of NFKBIZ compromised the effects of miR-4734, underscoring the importance of this direct targeting. As the effects of this miR-4734 were evident under stress, but not under basal conditions, it may be considered as a therapeutic agent.
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| Overall design |
For the mRNA expression array analysis, total RNA derived from miR-CTRL or miR-4734-transfected HFF were used.
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| Contributor(s) |
Michael D, Feldmesser E, Maman A, Oren M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 06, 2022 |
| Last update date |
Oct 10, 2023 |
| Contact name |
Ester Feldmesser |
| E-mail(s) |
ester.feldmesser@weizmann.ac.il
|
| Phone |
+972 8934 2614
|
| Organization name |
Weizmann Institute
|
| Department |
Life Science Core Facilities
|
| Street address |
POB 25
|
| City |
Rehovot |
| State/province |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE214963 |
A role for miR-4734 in conditional suppression of ER stress-associated pro-inflammatory responses |
|
| Relations |
| BioProject |
PRJNA887800 |
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