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| Status |
Public on Feb 18, 2011 |
| Title |
The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
A challenge in systems biology is to understand the gene regulatory networks that connect early cellular specification to terminal differentiation of specific cell types. In neurogenesis, neural specification has been well studied, but the link between the proneural transcriptional regulators of specification and the genes that must be activated to construct differentiated neurons is obscure. High resolution temporal profiling of gene expression reveals the events downstream of Atonal (Ato) proneural gene function in Drosophila sensory neurons. Unexpectedly, many differentiation genes are activated soon after specification, even before cell cycle exit and overt neuronal differentiation. Prominent among them are genes required for construction of the ciliary dendrite. Ato activates differentiation both directly and indirectly via several intermediate transcriptional regulators, including Rfx and a new Forkhead family factor. Our analysis of these factors and their regulation provides insight into how proneural factors regulate neuronal subtype differentiation.
Investigating the molecular mechanisms of peripheral nervous sytem development in Drosophila melanogaster.
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| Overall design |
Affymetrix Drosophila version 2.0 chips used to measure gene expression from GFP+ and GFP- cells from embryos expressing GFP under the control of the atonal gene enhancer in both wild type and mutant embryos. Data generated for three developmental time-points in quadruplicate.
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| Contributor(s) |
Simpson TI, Cachero S, ZurLage PI, Jarman AP |
| Citation(s) |
21283833 |
| Submission date |
Apr 26, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ian Simpson |
| URL |
http://neuroregulatorygenomics.org
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| Organization name |
University of Edinburgh
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| Department |
Institute for Adaptive and Neural Computation
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| Lab |
School of Informatics
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| Street address |
10 Crichton Street
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| City |
Edinburgh |
| ZIP/Postal code |
EH8 8AB |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA125961 |
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