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| Status |
Public on Feb 10, 2023 |
| Title |
Defining the effects of Rpl3L-deficiency on transcriptional profiles of mouse hearts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The ribosomal protein L3-like (RPL3L) is a striated muscle-specific ribosomal protein, and mutations in human RPL3L are linked with childhood cardiomyopathy and age-related atrial fibrillation. However, the function served by this protein, a paralogue of the ubiquitously expressed RPL3 protein, remains poorly characterized in both heart and skeletal muscle. To address this, null mutant mice with the Rpl3L gene deleted were generated and studied. The purpose of this microarray analyses was to compare transcriptional profiles of the null mutant hearts (lacking RPL3L) with wild-type control hearts (expressing RpL3L).
We used microarrays to elucidate effects of Rpl3L deficiency on transcriptional profile of mouse hearts
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| Overall design |
Hearts were harvested from control and experimental mice on the C57/BL6 background. Total RNA was isolated using the Trizol protocol and submitted to Affymetrix Clariom S array pipeline after quality control analysis using Agilent 2100 Bioanalyzer.
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| Contributor(s) |
Grimes K, Prasad V, Molkentin JD |
| Citation(s) |
36733907 |
| Submission date |
Oct 20, 2022 |
| Last update date |
Feb 11, 2023 |
| Contact name |
Jeffery D Molkentin |
| E-mail(s) |
vik.jd.geosubmissions@gmail.com
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Department |
Molecular Cardiovascular Biology
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| Lab |
Molkentin Lab
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| Street address |
240 Albert Sabin Way MLC 7020
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| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA892477 |
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