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| Status |
Public on Nov 01, 2022 |
| Title |
Stress triggers expression of bovine herpesvirus 1 infected cell protein 4 (bICP4) RNA during early stages of reactivation from latency in pharyngeal tonsil |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bovine herpesvirus 1 (BoHV-1), an important pathogen of cattle, establishes lifelong latency in sensory neurons within trigeminal ganglia (TG) after acute infection. The BoHV-1 latency-reactivation cycle, like other -herpesvirinae subfamily members, is essential for viral persistence and transmission. Notably, cells within pharyngeal tonsil (PT) also support a quiescent or latent BoHV-1 infection. The synthetic corticosteroid dexamethasone, which mimics the effects of stress, consistently induces BoHV-1 reactivation from latency allowing early stages of viral reactivation to be examined in the natural host. Based on previous studies, we hypothesized that stress-induced cellular factors trigger expression of key viral transcriptional regulatory genes. To explore this hypothesis, RNA-sequencing studies compared viral gene expression in PT during early stages of dexamethasone-induced reactivation from latency. Strikingly, RNA encoding infected cell protein 4 (bICP4), which is translated into an essential viral transcriptional regulatory protein, was detected 30 minutes after dexamethasone treatment. Ninety minutes after dexamethasone treatment bICP4 and to a lesser extent bICP0 RNA were detected in PT. All lytic cycle viral transcripts were detected within three hours after dexamethasone treatment. Surprisingly, the latency related (LR) gene, the only viral gene abundantly expressed in latently infected TG neurons, was not detected in PT during latency. In TG neurons, bICP0 and the viral tegument protein VP16 are expressed before bICP4 during reactivation suggesting distinct viral regulatory genes mediate reactivation from latency in PT versus TG neurons. Finally, these studies confirm PT is a biologically relevant site for BoHV-1 latency, reactivation from latency, and virus transmission.
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| Overall design |
We performed RNA-sequencing studies and compared viral gene expression in bovine pharyngeal tonsils (PT) using BoHV-1 latently infected (controls) and BoHV-1 genes in PT reactivated from latency during early timepoints (0-min 30-min, 90-min, 180-min) following dexamethasone treatment (Three biological replicates for each timepoint). We analyzed simultaneous expression of Bos taurus cellular genes.
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| Contributor(s) |
Toomer G, Workman A, Harrison KS, Stayton E, Hoyt PR, Jones C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 27, 2022 |
| Last update date |
Nov 01, 2022 |
| Contact name |
Clinton R. Jones |
| E-mail(s) |
clint.jones10@okstate.edu
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| Phone |
405-744-1842
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| Organization name |
Oklahoma State University
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| Department |
Veterinary Pathobiology
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| Street address |
250 McElroy Hall
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| City |
Stillwater |
| State/province |
Oklahoma |
| ZIP/Postal code |
74078-2007 |
| Country |
USA |
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| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
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| Samples (12)
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| GSM6685967 |
BoHV-1 infected Bos taurus peripheral tonsils without DEX treatment control,1 |
| GSM6685968 |
BoHV-1 infected Bos taurus peripheral tonsils without DEX treatment control, 2 |
| GSM6685969 |
BoHV-1 infected Bos taurus peripheral tonsils without DEX treatment control, 3 |
| GSM6685970 |
BoHV-1 infected Bos taurus peripheral tonsils 0.5hours post DEX treatment, 1 |
| GSM6685971 |
BoHV-1 infected Bos taurus peripheral tonsils 0.5hours post DEX treatment, 2 |
| GSM6685972 |
BoHV-1 infected Bos taurus peripheral tonsils 0.5hours post DEX treatment, 3 |
| GSM6685973 |
BoHV-1 infected Bos taurus peripheral tonsils 1.5 hours post DEX treatment, 1 |
| GSM6685974 |
BoHV-1 infected Bos taurus peripheral tonsils 1.5 hours post DEX treatment, 2 |
| GSM6685975 |
BoHV-1 infected Bos taurus peripheral tonsils 1.5 hours post DEX treatment, 3 |
| GSM6685976 |
BoHV-1 infected Bos taurus peripheral tonsils 3 hours post DEX treatment, 1 |
| GSM6685977 |
BoHV-1 infected Bos taurus peripheral tonsils 3 hours post DEX treatment, 2 |
| GSM6685978 |
BoHV-1 infected Bos taurus peripheral tonsils 3 hours post DEX treatment,3 |
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| Relations |
| BioProject |
PRJNA894969 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216685_gene_exp.diff.gz |
5.7 Mb |
(ftp)(http) |
DIFF |
| GSE216685_genes.count_tracking.txt.gz |
765.1 Kb |
(ftp)(http) |
TXT |
| GSE216685_genes.fpkm_tracking.gz |
1.1 Mb |
(ftp)(http) |
FPKM_TRACKING |
| GSE216685_genes.read_group_tracking.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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