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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 01, 2023 |
| Title |
Clariom S Microarray Expression Data from 4T1 parental and transduced tumors collected from WT and Nlrx1-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The immune signaling protein NLRX1 can be either tumor promoting or tumor suppressing in different models of cancer. We demonstrate that in a mammary tumor model of triple-negative breast cancer, NLRX1 impacts tumor volume and lung metastasis. We used this microarray to understand what genes and pathways are impacted by NLRX1 in murine triple-negative mammary tumors to produce the in vivo phenotypes we observed.
Abstract from associated publication: Prior studies have defined multiple, but inconsistent, roles for NLRX1 in regulating cancer-associated biological functions. Here, we explore the role of NLRX1 in the highly-metastatic murine 4T1 mammary tumor model. Using Nlrx1-/- mice engrafted with 4T1 tumors, we demonstrate that NLRX1 functions as a tumor suppressor when expressed in healthy host cells. Specifically, NLRX1 attenuates tumor growth and metastasis through suppressing epithelial-mesenchymal transition, tumor-associated eosinophil recruitment, and the lung metastatic niche. Conversely, we demonstrate that NLRX1 functions as a tumor promoter when expressed in 4T1 tumor cells using gain- and loss-of-function studies. Mechanistically, NLRX1 augments 4T1 aggressiveness through regulating epithelial-mesenchymal transition, cell death, proliferation, migration, ROS levels, and mitochondrial respiration. Together, we provide critical insight into NLRX1 function in breast cancer and establish cellular context as the director of the dichotomous role of NLRX1 in mammary tumor metastasis.
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| Overall design |
12 pooled RNA samples extracted from murine 4T1 (murine breast cancer) tumors. Comparing gene expression between 6 groups, consisting of 2 mouse genotypes (WT, KO) and 3 4T1 cell lines (4T1, 4T1OE, 4T1OECTL). 6 groups x 2 replicates each = 12 total samples. 1 MRSL, provided by the manufacturer, was included. Based on PCA analysis, ThermoFisher Clariom S service recommended excluding sample A3 from analysis.
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| Contributor(s) |
Nagai-Singer MA, Allen IC |
| Citation(s) |
37909827 |
| Submission date |
Oct 31, 2022 |
| Last update date |
Nov 12, 2023 |
| Contact name |
Irving Coy Allen |
| E-mail(s) |
icallen@vt.edu
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| Organization name |
Virginia Tech
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| Street address |
295 Duck Pond Dr
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| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA896210 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216937_RAW.tar |
17.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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