|
| Status |
Public on Jul 07, 2011 |
| Title |
Gene expression profiling of the Se-Ax cell line following E2A reconstitution |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcription factor E2A is essential for lymphocyte development. In this study, we describe a recurrent E2A gene deletion in at least 70% of patients with Sézary syndrome (SS), a subtype of T cell lymphoma. Loss of E2A results in enhanced proliferation and cell cycle progression via derepression of the proto-oncogene MYC and the cell cycle regulator CDK6. Furthermore, by examining the gene expression profile of SS cells following restoration of E2A expression, we identify a number of E2A-regulated genes that interfere with oncogenic signaling pathways including the Ras pathway. Several of these genes are down-regulated or lost in primary SS tumor cells. These data demonstrate a tumor suppressor function of E2A in human lymphoid cells and could help to develop new treatment strategies for human lymphomas with altered E2A activity.
|
| |
|
| Overall design |
The E2A-deficient Sézary cell line Seax was transiently transfected with the E2A-expression constructs E47myc (a myc-tagged E47 construct) or E47-forced dimer (a construct coding for two covalently linked E47 molecules), respectively. Every experiment was performed as two replicates accompanied by control transfections with a Mock plasmid.
|
| |
|
| Contributor(s) |
Köchert K, Mathas S, Steininger A, Ullmann R |
| Citation(s) |
21788410 |
| Submission date |
May 07, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Reinhard Ullmann |
| E-mail(s) |
ullmann@molgen.mpg.de
|
| Phone |
00493084131251
|
| Organization name |
MPIMG
|
| Department |
Human Molecular Genetics
|
| Lab |
Molecular Cytogenetics
|
| Street address |
Ihnestr.73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE21731 |
Genomic loss of the putative tumor suppressor gene E2A promotes cutaneous T-cell lymphoma in human |
|
| Relations |
| BioProject |
PRJNA129381 |
Less...
More...