|
| Status |
Public on Apr 01, 2011 |
| Title |
Nucleosome map of the IMR90 fibroblast cell line |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We produced a map of nucleosome positions in IMR90 by sequencing the ends of MNase-digested chromatin fragments.
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| |
|
| Overall design |
IMR90 cells were grown in culture, about 1E6 cells were isolated and digested using micrococcal nuclease (MNase). Mononucleosomes were gel-selected and fragment ends were sequenced using the Illumina GAIIx sequencing platform.
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| |
|
| Contributor(s) |
Berman BP, Kelly TK, Jones PA, Laird PW |
| Citation(s) |
22960375 |
| Submission date |
May 13, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Benjamin Berman |
| E-mail(s) |
bberman@usc.edu
|
| Organization name |
University of Southern California
|
| Department |
USC Epigenome Center
|
| Street address |
1450 Biggy St. #G511
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (1) |
|
| Relations |
| SRA |
SRP002541 |
| BioProject |
PRJNA127079 |
