|
| Status |
Public on Oct 03, 2023 |
| Title |
Gene expression analysis of Vg4+ vs Vg5/8/9+ gd T cells isolated from human gut |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The aim of this experiment was to characterize the the gene expression profile of colonic Vg4+ cells and to compare it to that of Vg5/8/9+ tissue-resident gd T cells.
|
| |
|
| Overall design |
Colonic biopsies (11 per donor) from 4 donors with no intestinal pathologies were dissociated with Miltenyi Biotec's tumour dissociation kit and stained with anti-Vg4 and anti-Vg2/3/4 antibodies. Vg4+Vg2/3/4+ (termed Vg4+) and Vg4-Vg2/3/4- (termed Vg5/8/9+) cells were flow sorted directly into lysis buffer and mRNA was used for library preparation and pair-end (PE100) sequencing on on Illumina NextSeq 2000.
|
| |
|
| Contributor(s) |
Zlatareva I, Hayday A, Kannambath S |
| Citation(s) |
37708268 |
| Submission date |
Nov 21, 2022 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Adrian Hayday |
| Organization name |
King's College London
|
| Department |
Immunobiology
|
| Street address |
Great Maze Pond
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA903871 |
Less...
More...