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Series GSE218617 Query DataSets for GSE218617
Status Public on Jul 04, 2023
Title Precision RNAi using synthetic shRNAmir target sites
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient, inducible, and reversible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects.
 
Overall design EGFRdel19::dsRed::ARTi engineered PC-9 cells harboring the Dox-inducible ARTi-shRNAmir were treated with Dox to elicit the knock-down of the EGFR transgene. KRASG12R-ARTi engineered MIA PaCa2 cells harboring the Dox-inducible ARTi-shRNAmir were treated with Dox to elicit the knock-down of the KRAS transgene. Differences in gene expression were monitored.
 
Contributor(s) Neumüller R, Popa A, Hörmann A, Köferle A
Citation(s) 37552050
Submission date Nov 23, 2022
Last update date Oct 03, 2023
Contact name Alexandra Popa
E-mail(s) alexandra.popa@boehringer-ingelheim.com
Organization name Boehringer Ingelheim
Street address Doktor-Boehringer-Gasse 5-11
City Vienna
State/province Austria
ZIP/Postal code 1120
Country Austria
 
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (60)
GSM6753297 PC9_EGFR_RN108RN76_doxycycline_8d_3
GSM6753298 PC9_EGFR_RN108RN76_untreated_8d_3
GSM6753299 PC9_EGFR_RN76_doxycycline_8d_3
Relations
BioProject PRJNA904685

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE218617_ARTi_MIAPaCa2_PC9_BI_normCounts.txt.gz 8.2 Mb (ftp)(http) TXT
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