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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 24, 2023 |
| Title |
CRISPR-based epigenome editing screens identify transcriptional and epigenetic regulators of human CD8 T cell function [ATAC-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Human CD8+ T cells were transduced with lentivirus encoding for HER2-CAR-2A-GFP or HER2-CAR-2A-BATF3.
T cells were either only stimulated once after thawing (acute stimulation) or repeatedly stimulated with HER2+ SKBR3 cells at a 1:2 effector to target (E:T) ratio every 3 days (chronic stimulation).
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| Overall design |
Comparative chromatin accessibility analysis of ATAC-seq data of acutely (n = 3) and chronically (n = 2) stimulated CD8+ T cells with or without BATF3 overexpression.
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| Contributor(s) |
McCutcheon SR, Gersbach CA |
| Citation(s) |
37205457, 37945901 |
| Submission date |
Nov 29, 2022 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Sean McCutcheon |
| E-mail(s) |
sean.mccutcheon@duke.edu
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| Phone |
7073447178
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| Organization name |
Duke University
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| Department |
Biomedical Engineering
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| Lab |
Charles Gersbach
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| Street address |
101 Science Drive, CIEMAS Rm. 2323
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
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| Platforms (1) |
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| Samples (10)
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| GSM6761476 |
CD8 T cells, GFP control, acute, donor 1, day 14 |
| GSM6761477 |
CD8 T cells, GFP control, acute, donor 2, day 14 |
| GSM6761478 |
CD8 T cells, GFP control, acute, donor 3, day 14 |
| GSM6761479 |
CD8 T cells, BATF3, acute, donor 1, day 14 |
| GSM6761480 |
CD8 T cells, BATF3, acute, donor 2, day 14 |
| GSM6761481 |
CD8 T cells, BATF3, acute, donor 3, day 14 |
| GSM6761482 |
CD8 T cells, GFP control, chronic, donor 1, day 14 |
| GSM6761483 |
CD8 T cells, GFP control, chronic, donor 2, day 14 |
| GSM6761484 |
CD8 T cells, BATF3, chronic, donor 1, day 14 |
| GSM6761485 |
CD8 T cells, BATF3, chronic, donor 2, day 14 |
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| This SubSeries is part of SuperSeries: |
| GSE218988 |
CRISPR-based epigenome editing screens identify transcriptional and epigenetic regulators of human CD8 T cell function |
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| Relations |
| BioProject |
PRJNA906521 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE218987_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of BW, NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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