|
| Status |
Public on Apr 19, 2023 |
| Title |
A senescent cell population with ZEB1 transcription factor as its main regulator promotes osteoarthritis in cartilage and meniscus [TC28_ZEB1_DRUGseq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the role of ZEB1 in chondrocytes, we transfected an overexpression vector containing cDNA for ZEB1 (Sino Biological; HG17705-UT) into TC28a2 cells using Lipofectamine 3000. We then performed DRUG-seq analysis to determine the impact of ZEB1 on global gene expression.
|
| |
|
| Overall design |
DRUG-seq (high throughput low-cost RNA-Seq) analysis of ZEB1 overexpression in chondrocyte cell line (TC28a2)
|
| |
|
| Contributor(s) |
Swahn H, Li K, Duffy T, Olmer M, D'Lima DD, Mondala TS, Natarajan P, Head SR, Lotz MK |
| Citation(s) |
36564153 |
| Submission date |
Dec 05, 2022 |
| Last update date |
Jul 20, 2023 |
| Contact name |
Padma Natarajan |
| E-mail(s) |
ccbb@scripps.edu
|
| Organization name |
The Scripps Research Institute
|
| Department |
CCBB
|
| Street address |
10550 N.Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (6)
|
| GSM6784587 |
TC28a2 cells transfected with control vector, replicate 1 [TC28_ZEB1_DRUGseq] |
| GSM6784588 |
TC28a2 cells transfected with control vector, replicate 2 [TC28_ZEB1_DRUGseq] |
| GSM6784589 |
TC28a2 cells transfected with control vector, replicate 3 [TC28_ZEB1_DRUGseq] |
| GSM6784590 |
TC28a2 cells transfected with ZEB1 overexpression vector, replicate 1 |
| GSM6784591 |
TC28a2 cells transfected with ZEB1 overexpression vector, replicate 2 |
| GSM6784592 |
TC28a2 cells transfected with ZEB1 overexpression vector, replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE220244 |
A senescent cell population with ZEB1 transcription factor as its main regulator promotes osteoarthritis in cartilage and meniscus |
|
| Relations |
| BioProject |
PRJNA908757 |
Less...
More...