|
| Status |
Public on Nov 09, 2023 |
| Title |
Abnormal Immune Programming in Neutrophils of Cystic Fibrosis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study performed single-cell RNA sequencing (scRNA-seq) analyses on peripheral blood neutrophils from cystic fibrosis (CF) patients and healthy controls (HC) in their native state and LPS-activated state. Differences in global gene transcription were interrogated and compared. Results demonstrate that CFTR loss of function in CF neutrophils led to abnormal immune programming, exemplified by precocious priming at their native state, and abnormal immune response to LPS.
|
| |
|
| Overall design |
Peripheral blood (~10 cc) from cystic fibrosis patients and healthy controls was drawn using a BD EDTA-purple top Vacutainer tube. Neutrophils were isolated by Percoll-density gradient centrifugation method, and were split into two aliquots. One was immediately processed for sc-RNA-seq, and the other was cultured and stimulated with Pseudomonas aeruginosa LPS (40 µg/ml) for 16 hours, followed by scRNA-seq.
|
| |
|
| Contributor(s) |
Wang G, Hu Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 10, 2022 |
| Last update date |
Nov 09, 2023 |
| Contact name |
Guoshun Wang |
| E-mail(s) |
gwang@lsuhsc.edu
|
| Phone |
5045687908
|
| Organization name |
Louisiana State University Health Sciences Center
|
| Street address |
CSRB 607, 533 Bolivar Street
|
| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA910830 |
Less...
More...