 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 20, 2024 |
| Title |
Role of MYC acetylated lysine residues in cell transformation and gene-selective regulation [Rat1A] |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The functions of MYC acetylation have remained unclear. In this study we identified the major lysine residues of MYC that are acetylated by p300 and GCN5 in cancer cell lines and established the requirment of three specific acetyl-lysine (AcK) residues for MYC transformation of Rat1a fibroblasts and MCF10 mammary epithelial cells. We further uncovered gene-selective regulatory functions and conserved genetic programs and biolofical processes regulated by MYC AcK residues in both cell types.
|
| |
|
| Overall design |
We generated stable cell lines (Rat1a) expressing empty vector, mouse MYC wild type (WT), MYC K149R (R149), MYC K158R (R158), or MYC K323R (R323). We performed stranded mRNA sequencing (mRNA-seq) on three biological replicates for each cell line.
|
| |
|
| Contributor(s) |
Hurd M, Gates R, Hamilton M, Girke T, Martinez E |
| Citation(s) |
37852796 |
| Submission date |
Dec 14, 2022 |
| Last update date |
Mar 21, 2024 |
| Contact name |
Ernest Martinez |
| E-mail(s) |
ernest.martinez@ucr.edu
|
| Organization name |
University of California Riverside
|
| Department |
Biochemistry
|
| Street address |
900 University Ave.
|
| City |
Riverside |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (15)
|
| GSM6829345 |
Rat1A Cells, Wild Type, rep1 |
| GSM6829346 |
Rat1A Cells, Wild Type, rep2 |
| GSM6829347 |
Rat1A Cells, Wild Type, rep3 |
| GSM6829348 |
Rat1A Cells, Mutant R149, rep1 |
| GSM6829349 |
Rat1A Cells, Mutant R149, rep2 |
| GSM6829350 |
Rat1A Cells, Mutant R149, rep3 |
| GSM6829351 |
Rat1A Cells, Mutant R158, rep1 |
| GSM6829352 |
Rat1A Cells, Mutant R158, rep2 |
| GSM6829353 |
Rat1A Cells, Mutant R158, rep3 |
| GSM6829354 |
Rat1A Cells, Mutant R323, rep1 |
| GSM6829355 |
Rat1A Cells, Mutant R323, rep2 |
| GSM6829356 |
Rat1A Cells, Mutant R323, rep3 |
|
| This SubSeries is part of SuperSeries: |
| GSE220920 |
Role of MYC acetylated lysine residues in cell transformation and gene-selective regulation |
|
| Relations |
| BioProject |
PRJNA912074 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220919_RAW.tar |
1.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...