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| Status |
Public on Dec 01, 2010 |
| Title |
Gene expression profiling of the early-stage CD34+ differentiation process |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by SAGE
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| Summary |
To better understand the early events regulating lineage-specific hematopoietic differentiation, we analyzed the transcriptional profiles of CD34+ human hematopoietic stem and progenitor cells (HSPCs) subjected to differentiation stimulus.
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| Overall design |
CD34+ cells were cultured for 12 and 40 hours in liquid cultures with supplemented media favoring myeloid or erythroid commitment. Serial analysis of gene expression (SAGE) was employed to generate four independent libraries. CD34+ Hematopoietic Stem Progenitor Cells with no differentiation stimulus were used as a control library.
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| Contributor(s) |
Molfetta GA, Zanette DL, Pinheiro DG, Panepucci RA, Silva WA Jr, Zago MA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 02, 2010 |
| Last update date |
Apr 18, 2012 |
| Contact name |
Daniel Guariz Pinheiro |
| E-mail(s) |
dgpinheiro@usp.br
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| Phone |
+55-16-2101-9300
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| Organization name |
Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo)
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| Department |
Departamento de Genética
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| Lab |
Laboratório de Genética Molecular e Bioinformática
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| Street address |
Rua Tenente Catão Roxo, 2501
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| City |
Ribeirão Preto |
| State/province |
São Paulo |
| ZIP/Postal code |
14051-140 |
| Country |
Brazil |
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| Platforms (1) |
| GPL4 |
SAGE:10:NlaIII:Homo sapiens |
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| Samples (3) |
| GSM549178 |
INCTC_Bone_marrow_normal_CD34+ |
| GSM549179 |
INCTC_Bone_marrow_normal_CD34+_Myeloid_12h |
| GSM549180 |
INCTC_Bone_marrow_normal_CD34+_Myeloid_40h |
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| Relations |
| BioProject |
PRJNA128827 |
