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| Status |
Public on Jan 01, 2011 |
| Title |
Role of retinoblastoma protein (Rb) in mouse Sertoli cells function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling study of Sertoli cells (Sc)-specific Retinoblastoma (Rb) knockout (KO) mice. We generated a Sc-specific Rb knockout (Sc-RbKO) line and characterised its testicular phenotype. Sc-RbKO showed a progressive germ cell depletion leading to infertility and Sc dysfunction. We observed that up to 10 days old, Sc-RbKO mice present a phenotype similar to that of their WT siblings. This age was choosen to study the gene expression changes that would eventually lead to the described phenotypical changes. Sc-RbKO testis gene expression was compared to WT and to Sc-Rb+/- samples. Our Microarray results show changes of many genes involved in cell cycle control, DNA repair, apoptosis and misregulation of genes that may be important for proper Sertoli cell function such as tight junction formation, cell membrane and tissue remodeling.
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| Overall design |
Total RNA extracted from 12 testicular samples, 5 controls, 3 hetorozygotes and 4 homozygotes.
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| Contributor(s) |
Bourguiba-Hachemi S, Rotgers E, Rivero-Muller A, Nurmio M, Parvinen M, Pelliniemi L, Toppari J, Da Ros M |
| Citation(s) |
24901045 |
| Submission date |
Jun 08, 2010 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Sonia Bourguiba-Hachemi |
| E-mail(s) |
sonia.bourguiba@utu.fi
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| Phone |
+35823337297
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| Fax |
+35822502610
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| Organization name |
University of Turku
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| Department |
Dept Physiology
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| Street address |
Kiinamyllynkatu 10
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| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA128847 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22234_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE22234_non-normalized.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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