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Status |
Public on Apr 19, 2023 |
Title |
Arabidopsis HOT3/eIF5B1 constrains rRNA RNAi by facilitating 18S rRNA maturation during translation initiation (RNA-Seq) |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Ribosome biogenesis is essential for protein synthesis in gene expression. Yeast eIF5B has been shown biochemically to facilitate 18S rRNA 3’ end maturation during late-40S ribosomal subunit assembly and gate the transition from translation initiation to elongation. But the effects of eIF5B have not been studied at the genome-wide level in any organism, and 18S rRNA 3’ end maturation is poorly understood in plants. Arabidopsis HOT3/eIF5B1 was found to promote development and heat-stress acclimation by translational regulation, but its molecular function remained unknown. Here, we show that HOT3 is a late-stage ribosome biogenesis factor that facilitates 18S rRNA 3’ end processing and is a translation initiation factor that globally impacts the transition from initiation to elongation. By developing and implementing 18S-ENDseq, we revealed previously unknown events in 18S rRNA 3’ end maturation or metabolism. We quantitatively defined new processing hotspots and identified adenylation as the prevalent non-templated RNA modification at the 3’ ends of pre-18S rRNAs. Aberrant 18S rRNA maturation in hot3 further activated RNAi to generate RDR1- and DCL2/4-dependent risiRNAs mainly from a 3’ portion of 18S rRNA. We further showed that risiRNAs in hot3 were predominantly localized in ribosome-free fractions not responsible for the 18S rRNA maturation or translation initiation defects in hot3. Our study uncovered the molecular function of HOT3/eIF5B1 in 18S rRNA maturation at the late-40S assembly stage and revealed the regulatory crosstalk among ribosome biogenesis, mRNA translation initiation, and siRNA biogenesis in plants.
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Overall design |
Comparative gene expression profiling analysis of RNA-seq data for 18-day-old seedlings of WT, hot3-2, and hot3-3
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Contributor(s) |
Hang R, You C, Chen X |
Citation(s) |
37011204 |
Submission date |
Jan 18, 2023 |
Last update date |
Apr 21, 2023 |
Contact name |
Chenjiang You |
E-mail(s) |
chenjiang.you@ucr.edu, youchenjiang2003@hotmail.com
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Organization name |
Fudan University
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Street address |
2005 Songhu Rd
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City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200438 |
Country |
China |
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Platforms (1) |
GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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Samples (7)
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This SubSeries is part of SuperSeries: |
GSE223172 |
Arabidopsis HOT3/eIF5B1 constrains rRNA RNAi by facilitating 18S rRNA maturation during translation initiation |
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Relations |
BioProject |
PRJNA925171 |