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Series GSE223761 Query DataSets for GSE223761
Status Public on Feb 12, 2023
Title Genome-wide RNAseq from multi time point (every 4 hours) rat hippocampi with vehicle and methylprednisolone (MPL) treatment
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Disrupted circadian activity is associated with many neuropsychiatric disorders. A major coordinator of circadian biological systems is adrenal glucocorticoid secretion which exhibits a pronounced pre-awakening peak that regulates metabolic, immune, and cardiovascular processes, as well as mood and cognitive function. Loss of this circadian rhythm during corticosteroid therapy is often associated with memory impairment. Surprisingly the mechanisms that underlie this deficit are not understood. In this study, in rats, we report that circadian regulation of the hippocampal transcriptome integrates crucial functional networks that link corticosteroid-inducible gene regulation to synaptic plasticity processes via an intra-hippocampal circadian transcriptional clock. Further, these circadian hippocampal functions were significantly impacted by corticosteroid treatment delivered in a five day oral dosing treatment protocol. Rhythmic expression of the hippocampal transcriptome, as well as the circadian regulation of synaptic plasticity were misaligned with the natural light/dark circadian entraining cues, resulting in memory impairment in hippocampal-dependent behavior. These findings provide mechanistic insights into how the transcriptional clock machinery within the hippocampus is influenced by corticosteroid exposure, leading to adverse effects on critical hippocampal functions, as well as identifying a molecular basis for memory deficits in patients treated with long-acting synthetic corticosteroids.  
 
Overall design Hippocampus RNA expression from adult CTL and methylprednisolone treated rats at zeitgeiber (ZT) 2,6,10,14,18,22
 
Contributor(s) Birnie MT, Lightman SL, Conway-Campbell BL
Citation(s) 37023133
Submission date Jan 26, 2023
Last update date May 03, 2023
Contact name Matthew Birnie
E-mail(s) mbirnie@uci.edu
Organization name University of California-Irvine
Street address Health Sciences Road
City Irvine
ZIP/Postal code 92617
Country USA
 
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (48)
GSM6994952 Untreated Hippocampus RNA ZT2_3807
GSM6994953 Untreated Hippocampus RNA ZT2_3808
GSM6994954 Untreated Hippocampus RNA ZT2_3809
Relations
BioProject PRJNA928340

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223761_FKPM_CTL_ZT10.txt.gz 223.5 Kb (ftp)(http) TXT
GSE223761_FKPM_CTL_ZT14.txt.gz 222.5 Kb (ftp)(http) TXT
GSE223761_FKPM_CTL_ZT18.txt.gz 221.7 Kb (ftp)(http) TXT
GSE223761_FKPM_CTL_ZT2.txt.gz 224.3 Kb (ftp)(http) TXT
GSE223761_FKPM_CTL_ZT22.txt.gz 220.8 Kb (ftp)(http) TXT
GSE223761_FKPM_CTL_ZT6.txt.gz 223.6 Kb (ftp)(http) TXT
GSE223761_FKPM_MPL_ZT10.txt.gz 226.0 Kb (ftp)(http) TXT
GSE223761_FKPM_MPL_ZT14.txt.gz 225.8 Kb (ftp)(http) TXT
GSE223761_FKPM_MPL_ZT18.txt.gz 224.9 Kb (ftp)(http) TXT
GSE223761_FKPM_MPL_ZT2.txt.gz 222.1 Kb (ftp)(http) TXT
GSE223761_FKPM_MPL_ZT22.txt.gz 224.8 Kb (ftp)(http) TXT
GSE223761_FKPM_MPL_ZT6.txt.gz 224.5 Kb (ftp)(http) TXT
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