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| Status |
Public on May 01, 2024 |
| Title |
Single cell ATAC-seq analysis of human dermal fibroblasts and human pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Human pluripotent stem cells have two major pluripotent states, primed and naive, and the heterogeneity among cell lines in each pluripotent state remains a major unresolved problem. We showed that the overexpression of H1FOO-DD, which has a short expression period by fusing the destabilized domain to the maternal-specific linker histone H1FOO, together with OCT4, SOX2, KLF4 and LMYC in human somatic cells improves the quality of reprogramming to primed and naive pluripotency.
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| Overall design |
Cultured human dermal fibroblasts and primed human pluripotent stem cell lines were sampled at specific time points, and analyzed by single cell ATAC-seq
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| Contributor(s) |
Kunitomi A, Kato TM, Nomura M, Oda M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 07, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Akira Kunitomi |
| E-mail(s) |
akira.kunitomi@gladstone.ucsf.edu
|
| Organization name |
The J. David Gladstone Institutes
|
| Street address |
1650 Owens Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE224850 |
H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency |
|
| Relations |
| BioProject |
PRJNA932225 |
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