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| Status |
Public on Jul 09, 2010 |
| Title |
Meningioma initiating cells (MICs) derived from primary tumors |
| Organism |
Homo sapiens |
| Experiment type |
SNP genotyping by SNP array
Genome variation profiling by SNP array
Expression profiling by array
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| Summary |
The majority of meningiomas are benign tumors associated with favorable outcomes; however, the less common aggressive variants with unfavorable outcomes often recur and may be due to sub-populations of less-differentiated cells residing within the tumor. These sub-populations of tumor cells, termed tumor-initiating cells, may be isolated from heterogeneous tumors when sorted or cultured in defined medium designed for enrichment of the tumor-initiating cells. We report the isolation and characterization of a population of tumor-initiating cells derived from an atypical meningioma. These meningioma-initiating cells (MICs) self-renew, differentiate, and can recapitulate the histological characteristics of the parental tumor when transplanted into athymic nude mice. Immunohistochemistry reveals protein expression patterns similar to neural stem and progenitor cells while genomic profiling verified the isolation of cancer cells (with defined meningioma chromosomal aberrations) from the bulk tumor. Furthermore, microarray analysis of gene expression reveals that many epithelial to mesenchymal transition genes are upregulated in the MICs, consistent with the presence of both neural stem cell and mature neural cell molecular markers seen in the derived cultures. Pathway analysis identifies biochemical processes and gene networks related to aberrant cell cycle progression, particularly the loss of heterozygosity of tumor suppressor genes CDKN2A (p16INK4A), p14ARF, and CDKN2B (p15INK4B). Flow cytometric analysis revealed the expression of CD44 and activated leukocyte adhesion molecule (ALCAM/CD166); these may prove to be markers able to identify this cell type. In conclusion, we identify a tumor-initiating population from an atypical meningioma that displays a unique phenotype and these results provide increased understanding of atypical meningioma progression.
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| Overall design |
Part 1 of 2: Affymetrix SNP arrays were performed according to the manufacturer's directions on DNA extracted from primary tissue and their counterpart cell lines
Part 2 of 2: Illumina gene expression array analysis was performed according to the manufacturer's directions on RNA extracted from cultured primary Meningioma and neural stem cell lines
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| Contributor(s) |
Shi H, Rath P, Free A |
| Citation(s) |
21168406 |
| Submission date |
Jun 25, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Huidong Shi |
| E-mail(s) |
hshi@augusta.edu
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| Phone |
706-721-6000
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| Organization name |
Augusta University
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| Department |
Georgia Cancer Center
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| Lab |
2125 K
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| Street address |
1120 15th Street, CN2138
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| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
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| Platforms (2) |
| GPL6804 |
[GenomeWideSNP_5] Affymetrix Genome-Wide Human SNP 5.0 Array |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (6)
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| GSM560319 |
1174 primary xenograft cell line in DN2L condition |
| GSM563827 |
1174 DN2L condition cell line (expression analysis) |
| GSM563828 |
NSPC DN2L condition cell line (expression analysis) |
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| Relations |
| BioProject |
PRJNA128409 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22577_RAW.tar |
108.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| GSE22577_non-normalized.txt.gz |
844.1 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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