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| Status |
Public on Jun 30, 2023 |
| Title |
MicroRNA-1289 functions as a novel tumor suppressor in oral squamous cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Recently, a number of tumor suppressive microRNAs (TS-miRs) have been found in human ma-lignancies. In this study, we have attempted to identify a novel TS-miR in oral squamous cell carcinoma (OSCC) using function-based screening. First, we transfected human OSCC cells with 968 synthetic mimicking human mature miRs individually, and then the growth of these cells was evaluated. Five miRs significantly reduced the cell growth rate by less than 30%, and miR-1289 had the most potent growth inhibitory effect among these miRs. Next, miR-1289/atelocollagen complex administration significantly reduced the size of subcutaneously xenografted human OSCC tumors. Furthermore, the expression levels of miR-1289 were significant decreased in OSCC tissues compared to adjacent normal oral mucosa. Finally, we identified magnesium transporter 1 as a target gene of miR-1289. These results suggest that miR-1289 functions as a novel TS-miR in OSCC, and may be a useful therapeutic tool for the patients with OSCC.
We explored the target genes of miR-1289 in human oral squamous cell carcinoma cells using microarray analysis.
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| Overall design |
We compared the gene expression profiles of 3 human oral squamous cell carcinoma cell lines treated with miR-1289 mimic and miR-non traget (NT).
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| Contributor(s) |
Nakashiro K |
| Citation missing |
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| Submission date |
Mar 09, 2023 |
| Last update date |
Jun 30, 2023 |
| Contact name |
Koh-ichi Nakashiro |
| E-mail(s) |
nakako@m.ehime-u.ac.jp
|
| Phone |
+81899605393
|
| Organization name |
Ehime University Graduate School of Medicine
|
| Department |
Oral and Maxillofacial Surgery
|
| Street address |
454 Shitsukawa
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
|
| Samples (6)
|
| GSM7089961 |
Transfection of miR-1289 mimic in GFP-SAS cells |
| GSM7089962 |
Transfection of miR-non target in GFP-SAS cells |
| GSM7089963 |
Transfection of miR-1289 mimic in Ca9-22 cells |
| GSM7089964 |
Transfection of miR-non target in Ca9-22 cells |
| GSM7089965 |
Transfection of miR-1289 mimic in HSC2 cells |
| GSM7089966 |
Transfection of miR-non target in HSC2 cells |
|
| Relations |
| BioProject |
PRJNA942904 |
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