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| Status |
Public on Jul 12, 2010 |
| Title |
Genes regulated by PCK Inhibitors, enzastaurin and Go, in CAL-27 cells, a SCCHN cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Efficacy of the Multi-Kinase Inhibitor Enzastaurin is Dependent on Cellular Signaling Context
Testing a panel of SCCHN cell lines revealed variable sensitivity to enzastaurin which correlated significantly with baseline cyclin D1 protein expression. Moreover, sensitivity and resistance could be reversed, respectively, by expression or depletion of cyclin D1. Furthermore, analysis of sensitive and resistant cell lines revealed distinct differences in cyclin D1 regulation. Enzastaurin modulated cyclin D1 synthesis via an AKT regulated pathway in the former while high level CCND1 gene amplification was present in the latter. These results underscore the critical relevance of cellular signaling context in developing cancer therapies, in general, and suggest that enzastaurin, in particular, would be most effective in tumors where baseline cyclin D1 expression is low to moderate and physiologically regulated.
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| Overall design |
CAL-27 cells were treated with the inhibitors for 24 hours and the gene expression from each group were analyzed
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| Contributor(s) |
Kuo W, Rosner M, Cohen E |
| Citation(s) |
20876745 |
| Submission date |
Jul 08, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Wen Kuo |
| E-mail(s) |
kuow@uchicago.edu
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| Phone |
773-702-4438
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| Organization name |
University of Chicago
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| Department |
Medicine/Hema/Onc
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| Lab |
ECohen
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| Street address |
KCBD 7220A 924 E. 57th St.
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA127901 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22811_RAW.tar |
33.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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