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| Status |
Public on Apr 29, 2024 |
| Title |
Senescent stroma mediates immunosuppression and drives breast cancer progression [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Tumor-bearing mammary fat pads from MMTV-PyMT mice were digested with 2mg/mL Collagenase A and 2U/mL Dnase.
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| Overall design |
FVB background MMTV-PyMT+/INK-ATTAC- (WT) and MMTV-PyMT+/INK-ATTAC+ (INK+) mice were weaned and recruited to studies by 28-30 days of age. Administration of AP20187 (10 mg/kg, 3 doses/week) was delivered through intra peritoneal inections . By 7 weeks of age, tumor-bearing mammary fat pads were digested to single cell suspension, staied, and sorted into CD45+ and CD45- populations. Samples from 2 mice were pooled for each sample.
FVB background MMTV-PyMT+ mice were weaned and recruited to studies by 28-30 days of age. Administration of ABT737 (25 mg/kg/week) or vehicle (Veh) was delivered through intra peritoneal inections . By 7 weeks of age, tumor-bearing mammary fat pads were digested to single cell suspension, staied, and sorted into CD45+ and CD45- populations. Samples from 2 mice were pooled for each sample.
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| Contributor(s) |
Ye J, Ren Q, Stewart SA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AG059244 |
MICROENVIRONMENTAL CONTROLS OF TUMOR DORMANCY |
WASHINGTON UNIVERSITY |
Sheila A Stewart |
| R01 CA217208 |
SENESCENT STROMA STIMULATES INFLAMMATION TO PROMOTE TUMORIGENESIS |
WASHINGTON UNIVERSITY |
Sheila A Stewart |
| T32 CA113275 |
Molecular Oncology Training Grant |
WASHINGTON UNIVERSITY |
Lee Ratner |
| F31 CA271721 |
Understanding how senescent stromal cells contribute to mammary gland tumorigenesis |
WASHINGTON UNIVERSITY |
Jiayu Ye |
| P30 CA091842 |
Cancer Center Support Grant |
WASHINGTON UNIVERSITY |
Timothy J Eberlein |
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| Submission date |
Apr 10, 2023 |
| Last update date |
Apr 29, 2024 |
| Contact name |
Sheila Stewart |
| E-mail(s) |
sheila.stewart@wustl.edu
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| Organization name |
Washington University School of Medicine
|
| Street address |
660 South Euclid Avenue
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| City |
Saint Louis |
| ZIP/Postal code |
314-362-7449 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE229356 |
Senescent stroma mediates immunosuppression and drives breast cancer progression |
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| Relations |
| BioProject |
PRJNA954052 |
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