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| Status |
Public on May 05, 2024 |
| Title |
Multicellular liver organoid model for recapitulating hepatitis C virus infection and non-alcoholic fatty liver disease progression [scRNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hepatitis C virus (HCV) infection has been successfully managed by anti-viral therapies, however, high prevalence to severe chronic liver disease state including non-alcoholic fatty liver disease (NAFLD) is a problem encountered even after the cure of hepatitis C. Moreover, there is currently no reliable in vitro model capable of investigating host-viral interactions and monitoring the progression of viral hepatitis to chronic liver diseases. Recent organoid technology has been reported for successful infection of HCV, but there is still lack of non-parenchymal cells, which play a crucial role in disease progression. Here, we provided a novel multicellular liver organoid model using co-culture system of macrophages and liver organoids differentiated from the same cell source, human embryonic stem cells. Surprisingly, HCV infection led potent lipid accumulation in liver organoids through regulation of host lipid metabolism, which was further promoted by macrophage co-culture. Lipid enriched condition provided by longterm-treatment with fatty acid accelerated potent HCV amplification and further promotion of inflammation and fibrosis progression. Therefore, our model may be a valuable novel platform recapitulating diverse phenotypes with host-virus intercommunication and inter-cellular interactions and further progressive features of hepatitis C-associated chronic NAFLD progression of patients with HCV.
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| Overall design |
Liver organoids were cultured without or with macrophages and analyzed using scRNAseq.
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| Contributor(s) |
Gil D, Lee J, Son MJ, Kim J |
| Citation missing |
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| Submission date |
Apr 13, 2023 |
| Last update date |
May 06, 2024 |
| Contact name |
Dayeon Gil |
| E-mail(s) |
gilje@korea.kr
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| Phone |
+82-43-249-2527
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| Organization name |
Korea national institute of health
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| Department |
Division of intractable disease research
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| Street address |
202 Osongsaengmyung 2-ro, Heungdeok-gu
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| City |
Cheongju-si |
| State/province |
Chungcheongbuk-do |
| ZIP/Postal code |
28160 |
| Country |
South Korea |
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| Platforms (1) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE229609 |
Multicellular liver organoid model for recapitulating hepatitis C virus infection and non-alcoholic fatty liver disease progression |
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| Relations |
| BioProject |
PRJNA955148 |
