|
| Status |
Public on May 15, 2024 |
| Title |
yHSC and oHSC 10X scRNAseq under ad lib, fasted, or fast refeed conditions to evaluate transcriptional impact of transient autophagy engagement in hematopoietic stem cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We observed that 24hr of fasting followed by a 24hr refeeding period improved the regenerative capacity of hematopoietic stem cells isolated from old mice, which was associated with an increase in glycolytic capacity. We sought to evaluate transcriptional changes in yHSC and oHSC compartment following different mouse feeding paradigms at single cell resolution. In yHSCs, AL and F/R groups showed overlapping transcriptional signatures with a distinct pattern for the fasted group. In contrast, in oHSCs, AL and fasted groups showed a strong overlap with a distinct transcriptional signature for the F/R group, reflective of differences in metabolic set points with age.
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| |
|
| Overall design |
Three young and three old mice were analyzied (one per feeding condition - Ad lib, 24hr Fast, 24hr Fast 24hr Refeed)
|
| |
|
| Contributor(s) |
Dellorusso P, Passegue E |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 21, 2023 |
| Last update date |
May 15, 2024 |
| Contact name |
Paul Vincent Dellorusso |
| E-mail(s) |
paul.dellorusso@gmail.com
|
| Phone |
5166552491
|
| Organization name |
Columbia University Irving Medical Center
|
| Department |
Genetics and Development
|
| Lab |
Passegue Lab
|
| Street address |
701 West 168th Street, 724 Hammer Health Sciences Center
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE229137 |
Autophagy engaging and non-engaging hematopoietic stem cells |
|
| Relations |
| BioProject |
PRJNA958196 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230284_c0vsRest_DEtable.txt.gz |
724.4 Kb |
(ftp)(http) |
TXT |
| GSE230284_c0vsRest_DEtable_c2Pathway.txt.gz |
131.5 Kb |
(ftp)(http) |
TXT |
| GSE230284_c10vsRest_DEtable.txt.gz |
695.5 Kb |
(ftp)(http) |
TXT |
| GSE230284_c10vsRest_DEtable_c2Pathway.txt.gz |
121.1 Kb |
(ftp)(http) |
TXT |
| GSE230284_c1vsRest_DEtable.txt.gz |
702.5 Kb |
(ftp)(http) |
TXT |
| GSE230284_c1vsRest_DEtable_c2Pathway.txt.gz |
129.9 Kb |
(ftp)(http) |
TXT |
| GSE230284_c2vsRest_DEtable.txt.gz |
715.5 Kb |
(ftp)(http) |
TXT |
| GSE230284_c2vsRest_DEtable_c2Pathway.txt.gz |
130.0 Kb |
(ftp)(http) |
TXT |
| GSE230284_c3vsRest_DEtable.txt.gz |
698.2 Kb |
(ftp)(http) |
TXT |
| GSE230284_c3vsRest_DEtable_c2Pathway.txt.gz |
128.3 Kb |
(ftp)(http) |
TXT |
| GSE230284_c4vsRest_DEtable.txt.gz |
703.2 Kb |
(ftp)(http) |
TXT |
| GSE230284_c4vsRest_DEtable_c2Pathway.txt.gz |
129.2 Kb |
(ftp)(http) |
TXT |
| GSE230284_c5vsRest_DEtable.txt.gz |
664.0 Kb |
(ftp)(http) |
TXT |
| GSE230284_c5vsRest_DEtable_c2Pathway.txt.gz |
121.4 Kb |
(ftp)(http) |
TXT |
| GSE230284_c6vsRest_DEtable.txt.gz |
709.1 Kb |
(ftp)(http) |
TXT |
| GSE230284_c6vsRest_DEtable_c2Pathway.txt.gz |
128.7 Kb |
(ftp)(http) |
TXT |
| GSE230284_c7vsRest_DEtable.txt.gz |
707.9 Kb |
(ftp)(http) |
TXT |
| GSE230284_c7vsRest_DEtable_c2Pathway.txt.gz |
127.3 Kb |
(ftp)(http) |
TXT |
| GSE230284_c8vsRest_DEtable.txt.gz |
701.0 Kb |
(ftp)(http) |
TXT |
| GSE230284_c8vsRest_DEtable_c2Pathway.txt.gz |
126.9 Kb |
(ftp)(http) |
TXT |
| GSE230284_c9vsRest_DEtable.txt.gz |
688.2 Kb |
(ftp)(http) |
TXT |
| GSE230284_c9vsRest_DEtable_c2Pathway.txt.gz |
123.5 Kb |
(ftp)(http) |
TXT |
| Raw data are available in SRA |
| Processed data are available on Series record |
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