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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 14, 2024 |
| Title |
Expression of the membrane tetraspanin claudin 18 on cancer cells promotes T lymphocyte infiltration and antitumor immunity in pancreatic cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Tumors weakly infiltrated by T lymphocytes poorly respond to immunotherapy. We aimed to unveil malignancy-associated programs regulating T cell entrance, arrest, and activation in the tumor environment. Differential expression of cell adhesion and tissue architecture programs, particularly the presence of the membrane tetraspanin claudin (CLDN)18 as a signature gene, demarcated immune-infiltrated from immune-depleted mouse pancreatic tumors. In human pancreatic ductal adenocarcinoma (PDAC) and non-small cell lung cancer, CLDN18 expression positively correlated with more differentiated histology and favorable prognosis. CLDN18 on the cell surface promoted accrual of cytotoxic T lymphocytes (CTLs), facilitating direct CTL contacts with tumor cells by driving the mobilization of the adhesion protein ALCAM to the lipid rafts of the tumor cell membrane through actin. This process favored the formation of robust immunological synapses (ISs) between CTLs and CLDN18-positive cancer cells, resulting in increased T cell activation. Our data reveal an immune role for CLDN18 in orchestrating T cell infiltration and shaping the tumor immune contexture.
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| Overall design |
Three biological replicates of FC1199-OVA and FC1242-OVA KPC-derived cell lines infected with ovalbumin and GFP-econding lentivirus
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| Contributor(s) |
De Sanctis F, Dusi S, Caligola S, Anselmi C, Petrova V, Rossi B, Angelini G, Erdeljan M, Wöll S, Schlitter M, Metzler T, Steiger K, Borok Z, Bailey P, Bauer A, Halin C, Boschi F, Giugno R, Canè S, Lawlor R, Corbo V, Scarpa A, Constantin G, Ugel S, Vascotto F, Sahin U, Tureci Ö, Bronte V |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 28, 2023 |
| Last update date |
May 15, 2024 |
| Contact name |
Francesco De Sanctis |
| E-mail(s) |
francesco.desanctis@univr.it
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| Organization name |
University and Hospital Trust of Verona
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| Department |
Department of Medicine
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| Street address |
Piazzale L.A. Scuro, 10
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| City |
Verona |
| ZIP/Postal code |
37134 |
| Country |
Italy |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA962882 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230868_RAW.tar |
20.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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