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| Status |
Public on May 05, 2024 |
| Title |
Diet-induced obesity dysregulates rapid epigenetic response to hypoxia in macrophages (ChIP-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Macrophages play important roles in tissue homeostasis and tissue healing process. The response to hypoxic environment is a trigger to induce key signaling pathways in macrophages. Functions of macrophages are dysregulated in metabolic diseases such as diabetes and obesity resulting in poor tissue healing. Epigenetic status including histone modification in macrophages is regulated by hypoxia. However, whether and how obesity influences histone modification under hypoxia is unknown. Using chromatin immunoprecipitation-sequencing (ChIP-seq), RNA-seq on mouse bone marrow-derived macrophages, we sought to determine whether hypoxia alters histone 3-lysine 4 trimethylation (H3K4me3) and gene expression profile in macrophages and how obesity influences H3K4me3 enrichment on the genome in hypoxia-dependent and -independent manner.
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| Overall design |
Comparative H3K4me3 marked peak analysis of ChIP-seq data for bone marrow-derived macrophages (BMDMs) obtained from C57B6/J mice fed a high-fat diet or a normal-fat diet with or without 1% hypoxia treatment for 1 hour.
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| Contributor(s) |
Urao N, Takahashi K |
| Citation missing |
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| Submission date |
May 08, 2023 |
| Last update date |
May 06, 2024 |
| Contact name |
Norifumi Urao |
| Organization name |
SUNY Upstate Medical University
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| Department |
Pharmacology
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| Street address |
750 E. Adams St.
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| City |
Syracuse |
| State/province |
New York |
| ZIP/Postal code |
13210 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA970368 |
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