|
| Status |
Public on Aug 09, 2010 |
| Title |
Chromatin Structure and Gene Expression Programs of Human Embryonic and Induced Pluripotent Stem Cells (ChIP-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Knowledge of both the global chromatin structure and the gene expression programs of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells should provide a robust means to assess whether the genomes of these cells have similar pluripotent states. Recent studies have suggested that ES and iPS cells represent different pluripotent states with substantially different gene expression profiles. We describe here a comparison of global chromatin structure and gene expression data for a panel of human ES and iPS cells. Genome-wide maps of nucleosomes with histone H3K4me3 and H3K27me3 modifications indicate that there is little difference between ES and iPS cells with respect to these marks. Gene expression profiles confirm that the transcriptional programs of ES and iPS cells show very few consistent differences. Although some variation in chromatin structure and gene expression was observed in these cell lines, these variations did not serve to distinguish ES from iPS cells.
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| |
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| Overall design |
Examination of two histone modifications (H3K4me3 and H3K27me3) in 6 human ES cell lines, 8 human iPS cell lines, and 1 fibroblast cell line.
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| |
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| Contributor(s) |
Guenther MG, Frampton GM, Soldner F, Hockemeyer D, Militipova M, Jaenisch R, Young RA |
| Citation(s) |
20682450, 22819920 |
| Submission date |
Aug 06, 2010 |
| Last update date |
Aug 23, 2019 |
| Contact name |
Garrett M Frampton |
| E-mail(s) |
frampton@mit.edu
|
| Organization name |
Whitehead Instute - MIT
|
| Department |
Biology
|
| Lab |
Richard Young
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (30)
|
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| This SubSeries is part of SuperSeries: |
| GSE22499 |
Chromatin Structure and Gene Expression Programs of Human Embryonic and Induced Pluripotent Stem Cells |
|
| Relations |
| SRA |
SRP003360 |
| BioProject |
PRJNA130981 |
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