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Series GSE235626 Query DataSets for GSE235626
Status Public on Jan 23, 2024
Title Association of inflammatory mediators with mitochondrial DNA mutations in geriatric COVID-19 patients
Organism Homo sapiens
Experiment type Genome variation profiling by genome tiling array
Summary This study aimed to evaluate single nucleotide substitutions in mtDNA and analyze their correlation with inflammatory biomarkers in elderly COVID-19 patients. A total of 30 COVID-19 patients and 33 older adult controls (aged over 65 years) were enrolled. mtDNA was extracted from buffy coat samples and sequenced using a chip-based resequencing system (Affymetrix MitoChip v2.0) which detects both homoplasmic and heteroplasmic mtDNA mutations, and allows the assessment of low-level heteroplasmy. Serum concentration of IL-6, IFN-α, TNF-α and IL-10 were determined in patients by a high-sensitivity immunoassay. We found a higher burden of total heteroplasmic mutation in COVID-19 patients compared to controls with a selective increment in ND1 and COIII genes. Low-level heteroplasmy was significantly elevated in COVID-19 patients, especially in genes of the respiratory complex I. Both heteroplasmic mutation burden and low-level heteroplasmy were associated with increased levels of IL-6, TNF-α, and IFN-α.
 
Overall design The study population comprised elderly individuals with COVID-19 (n=30, experimental group) and age-matched controls (n=33). Blood samples were collected from individuals who were admitted to the IRCCS INRCA Hospital, Ancona, Italy, and were enrolled in either the Report-Age COVID (experimental patients) or Report-Age (control subjects) projects, respectively. The aim of the Report-Age COVID project is to provide a deeper understanding of COVID-19 disease in elderly patients (≥ 65 years). The experimental subjects were COVID-19 positive cases as confirmed by the detection of SARS-CoV-2 RNA in nasal/oropharyngeal swabs while controls were COVID-19 negative elderly subjects. Demographic and anamnestic data, biochemical and hematological variables, information on treatments, comorbidities, and survival were collected in a retrospective manner and anonymized before release.
 
Contributor(s) Casoli T, Bonfigli AR, Di Rosa M
Citation(s) 38377022
Submission date Jun 22, 2023
Last update date Apr 24, 2024
Contact name Tiziana Casoli
E-mail(s) t.casoli@inrca.it
Phone +39 071 8004203
Organization name INRCA
Department Scientific Technological Area
Lab Center for Neurobiology of Aging
Street address Via Birarelli 8
City Ancona
ZIP/Postal code 60121
Country Italy
 
Platforms (1)
GPL10983 [Mitochip_2] Affymetrix Human Mitochondrial Resequencing Array 2.0
Samples (63)
GSM7506420 Human blood, COVID 1
GSM7506421 Human blood, COVID 2
GSM7506422 Human blood, COVID 3
Relations
BioProject PRJNA986577

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE235626_RAW.tar 123.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file
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