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Series GSE23730 Query DataSets for GSE23730
Status Public on Sep 18, 2012
Title Oncofusion protein AML1-ETO targets ERG factor binding sites in AML
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary ERG has been identified as an essential factor for the function and maintenance of adult hematopoietic stem cells and high ERG expression is a negative prognostic marker for treatment outcome in AML. The molecular function of ERG and its interplay with other factors is however largely unknown. Here we demonstrate that ERG has cell type specific distributions in normal CD34+ myeloid progenitors and in AML cells and identify ERG as a potential pioneering protein for binding of oncofusion protein complexes. In addition, we identify H3 acetylation as the epigenetic mark preferentially associated with ERG binding. This intimate connection between ERG binding and H3 acetylation implies that one of the molecular strategies of the oncofusion proteins PML-RARa and AML1-ETO could involve the targeting of histone deacetylase activities to ETS factor bound hematopoietic regulatory sites.
 
Overall design Examination of AML1-ETO, RUNX1, CBFb, HEB, FLI1 and ERG binding sites (ChIP-seq) in leukemic and normal hematopoietic cells, association with chromatin modifications and expression (RNA-seq) analysis of an AML1-ETO expressing cell line (SKNO-1)
 
Contributor(s) Stunnenberg HG, Martens JH, Mandoli A, Simmer F, Altucci L, Vellenga E, Wierenga B
Citation(s) 22983443
Submission date Aug 20, 2010
Last update date May 15, 2019
Contact name H.G. Stunnenberg
E-mail(s) h.stunnenberg@ncmls.ru.nl
Phone +31-24-3610520
Organization name Radboud University
Department Department of Molecular Biology (274)
Street address Geert Grooteplein 28
City Nijmegen
ZIP/Postal code 6525 GA
Country USA
 
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (42)
GSM585585 Kasumi-1_AE (A706_Diagenode)_no
GSM585586 Kasumi-1_HEB(SantaCruz_sc357_A20)_no
GSM585587 Kasumi-1_ETO1(A710_Diagenode)_no
Relations
SRA SRP003496
BioProject PRJNA130783

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Supplementary file Size Download File type/resource
GSE23730_RAW.tar 4.0 Gb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA
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