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Series GSE239815 Query DataSets for GSE239815
Status Public on Apr 03, 2024
Title Epigenetic inheritance of diet-induced and sperm-borne mitochondrial RNAs
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Spermatozoa harbor a complex and environment sensitive pool of small non-coding RNAs (sncRNA)1, which influences offspring development and adult phenotypes1-7. Whether spermatozoa in the epididymis are directly susceptible to environmental cues is not fully understood8. We used two distinct paradigms of preconception acute high fat diet to dissect epididymal vs testicular contributions to the sperm sncRNA pool and offspring health. We show that epididymal spermatozoa, but not developing germ cells, are sensitive to the environment and identify mitochondrial tRNAs and their fragments (mt-tsRNA) as sperm-borne factors. In humans, mt-tsRNAs in spermatozoa correlate with BMI and paternal overweight at conception doubles offspring obesity risk and compromises metabolic health. Sperm sncRNA-seq of mice mutant for genes involved in mitochondrial function, and metabolic phenotyping of their wild-type offspring, suggest that alterations of mt-tsRNAs are downstream of mitochondrial dysfunction. Most importantly, single embryo transcriptomics of genetically hybrid two-cell embryos demonstrated sperm-to-oocyte transfer of mt-tRNAs at fertilisation and implied them in the control of early embryo transcription. Our study supports the importance of paternal health at conception for offspring metabolism, shows that mt-tRNAs are diet-induced and sperm-borne and demonstrates, for the first time in a physiological setting, father-to-offspring transfer of sperm mitochondrial RNAs at fertilization.
 
Overall design Refer to individual Series
 
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Submission date Aug 01, 2023
Last update date Apr 04, 2024
Contact name Raffaele Teperino
E-mail(s) raffaele.teperino@helmholtz-muenchen.de
Organization name Helmholtz Zentrum München
Department Institute of Experimental Genetics (IEG)
Lab Environmental Epigenetics
Street address Ingolstädter Landstraße 1
City München
ZIP/Postal code 85764
Country Germany
 
Platforms (3)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (307)
GSM7674003 Morula LFD-1
GSM7674004 Morula LFD-2
GSM7674005 Morula LFD-3
This SuperSeries is composed of the following SubSeries:
GSE239807 Mitochondrial signals mediate paternal effects on offspring metabolism (RNA-Seq)
GSE239808 Mitochondrial signals mediate paternal effects on offspring metabolism (scRNA-Seq)
GSE239810 Mitochondrial signals mediate paternal effects on offspring metabolism (small RNA-Seq)
Relations
BioProject PRJNA1001005

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE239815_RAW.tar 1.7 Gb (http)(custom) TAR (of MTX, TSV, TXT)
SRA Run SelectorHelp

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