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| Status |
Public on Aug 25, 2017 |
| Title |
Dengue virus type-3 isolated from a fatal case with visceral complications induces potent inflammatory responses associated with apoptosis in human monocyte-derived dendritic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Dengue virus (DENV) infection is one of the most serious public health problems worldwide. A recent dengue outbreak in Paraguay (2007-2009) presented unusual manifestations such as hepatitis, encephalitis, pulmonary as well as cardiac disorders associated with 50% of deaths caused by dengue in the country. Despite the knowledge on inflammatory responses observed during the course of disease, the role of innate immune cells in the control of virus replication influencing clinical outcome is poorly defined. Using two clinical isolates of the virus, a non-fatal case of classical DF (DENV3/290) and a fatal case of DF with visceral complications (DENV3/5532), we sought to determine the profile of dengue infection in human dendritic cell, a major innate immune cell population. Compared to classical DENV3/290, the strain DENV3/5532 displayed higher replicative ability in mdDCs. In addition, DENV3/5532 was found to induce elevated production of pro-inflammatory cytokines associated with higher rates of programmed cell death. The observed phenotype was due to viral replication in mdDCs and TNF appeared to display a protective effect on virus-induced mdDCs apoptosis. These results suggest that the fatal case DENV3/5532 isolate modulates dendritic cell survival as well as inflammatory mediator’s synthesis.
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| Overall design |
In this study we analyzed the mRNA pool of 10 cultures. Each culture was infected with MOI of 5.0, and the cells were extracted at the times points of 06, 12, 24, and 48 hours after infection.
As infection control, the same 10 cultures were mock-infected. This mRNA pool is used to hibridizated 8 chips, with no replicates.
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| Contributor(s) |
Silveira GF, Meyer F, Delfraro A, Mosimann AP, Coluch N, Vasquez C, Probst CM, Báfica A, Bordignon J, Santos CN |
| Citation(s) |
21450836 |
| Submission date |
Sep 07, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Guilherme Ferreira Silveira |
| E-mail(s) |
gfsilveira@gmail.com
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| Organization name |
Instituto Carlos Chagas
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| Street address |
Prof Algacyr Munhoz Máder
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| City |
Curitiba |
| ZIP/Postal code |
81350-010 |
| Country |
Brazil |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA130483 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23986_RAW.tar |
34.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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