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| Status |
Public on May 15, 2024 |
| Title |
BALF single-cell landscape in CARDS and non-ARDS patients at two time points |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, single-cell sequencing of the BALF of CARDS patients yielded the following results: (1) IL1B+ neutrophils were significantly increased in the BALF of CARDS patients with exacerbated disease, which was associated with poor prognosis of CARDS patients, and this type of neutrophils underwent fatty acid metabolism reprogramming in comparison with other types of neutrophils, which may be related to their high-inflammatory response. (2) IL1B+ neutrophils promoted TNF secretion from hyperinflammatory type M1 macrophages through a caspase-dependent pathway, suggesting that neutrophil-macrophage interactions play an important role in the mechanism of CARDS development.
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| Overall design |
A total of 8 patients with severe ARDS, including 4 CARDS patients and 4 non-CARDS patients (2 bacterial pneumonia, 2 fungal pneumonia), were included in this study, of which 2 patients were treated with ECMO, and the rest of the patients received hormone therapy except for 1 patient who did not receive hormone therapy. BALF was retained in each patient at the time point of admission to the ICU (t1) and at the time point of development of an MS change value ≥1 point (t2).
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| Contributor(s) |
Yang Y, Li H, Liu P, Long Y, Su L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 10, 2023 |
| Last update date |
May 15, 2024 |
| Contact name |
Longxiang Su |
| E-mail(s) |
sulongxiang@gmail.com
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| Organization name |
Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science
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| Department |
Department of Critical Care Medicine
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| Street address |
1 Shuaifu Yuan
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1004268 |
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