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| Status |
Public on May 01, 2024 |
| Title |
Extracellular vesicles from epicardial fat mediate long-distance heart-brain communication and promote atrial fibrillation associated cognitive impairment (EAT exosomal miRNA-seq and hippocampal mRNA-seq) |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
2 weeks of rapid atrial pacing increased the release of epicardial adipose tissue (EAT) exosomes. The red fluorescence of Ad-CD63-RFP traces the increase of EAT exosomes in the canine hippocampus. RNA Sequencing showed that the differentially expressed genes of cfa-miR-22e in EAT exosomes were significantly increased, and its target gene of IL33 was down-regulated in the hippocampus. Exosomes secreted by EAT in AF model canine penetrate the BBB, and activate hippocampal microglia through cfa-miR-22e/IL33 signaling pathway, which may be related to the increased risk of cognitive impairment in AF.
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| Overall design |
To investigate the effect of atrial fibrillation on cognitive impairment, canines were randomly divided into the sham group, the pacing group. The miRNA was detected from EAT exosomes of canines in the sham group and the pacing group, and the mRNA was detected from hippocampal tissue in the same group of canines. The results of heart and brain sequencing were analyzed jointly
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| Contributor(s) |
Wang X, Ke Y, Cao Z, Zhao Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 14, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
wang xuewen |
| E-mail(s) |
wangxuewen@whu.edu.cn
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| Phone |
+8618372030240
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| Organization name |
Renmin Hospital of Wuhan University
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| Department |
Department of Cardiology
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| Lab |
Cardiovascular Research Institute
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| Street address |
238 Jiefang Road, Wuhan 430060, China
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| City |
Wuhan |
| State/province |
中国 (+86) |
| ZIP/Postal code |
430060 |
| Country |
China |
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| Platforms (1) |
| GPL33670 |
DNBSEQ-T7 (Canis lupus familiaris) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1005232 |
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