 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2011 |
| Title |
Hypoxia suppresses neuronal functions in primary neuron, speeds cell cycle progression and affection the differentiation potential of neural stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Multiple diseases are associated with a pathological hypoxia in the brain, resulting in various neurological sequalae. Understanding the response to hypoxia of neurons and neural stem cells (NSCs) will help devise better therapeutic strategies. We have exposed primary neurons (PN) and neural stem cells to 1% O2. Both cell types survived well, and neurons showed no obvious morphological changes. The NSCs, however, became fusiform, and displayed a population of cells with accelerated transition in cell cycle. Gene expression profile through microarray analysis revealed major differences in response to hypoxia between NSC and PN. Not only the number of genes significantly changes was ~five-fold higher in NSC, but the types of genes involved and the direction of change was quite different. In particular, NSCs up-regulated multiple growth factors and down-regulated most other cytokines and metalloproteases , while PN down-regulated most neuronal-specific genes, up-regulated growth factors, with no major effect on cytokines. We conclude that hypoxia 1- accelerates cell cycle transition of NSC in a post-transcriptional fashion ; 2-affects cytokines in NSC but not in neurons; 3-result in up-regulation of multiple growth factors in NSC and PN; and 4-suppresses neuronal specific functions.
|
| |
|
| Overall design |
1) Primary neurons (PN) and neural stem cells were exposed to 1% O2.
2) Gene expression profile through microarray analysis was used to determine the differences in response to hypoxia between NSC and PN.
|
| |
|
| Contributor(s) |
Felfly H, Zhou D, Xue J, Haddad GG |
| Citation |
Severe Hypoxia: Consequences to Neural Stem Cells and Neurons. Hady Felfly, Alexander C. Zambon, Jin Xue, Alysson Muotri, Dan Zhou, Evan Y. Snyder, Gabriel G. Haddad. Journal of Neurology Research, Vol. 1, No. 5, Dec 2011. doi:10.4021/jnr70w
|
| Submission date |
Sep 14, 2010 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Dan Zhou |
| E-mail(s) |
d2zhou@ucsd.edu
|
| Phone |
858-822-6889
|
| Fax |
858-534-6971
|
| Organization name |
University of California, San Diego, School of Medicine
|
| Department |
Pediatrics
|
| Lab |
Haddad Lab
|
| Street address |
CMG 103, 9500 Gilman Drive, MC0735
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0735 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA130125 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24131_RAW.tar |
464.6 Mb |
(http)(custom) |
TAR (of TIFF) |
| GSE24131_raw_data.txt.gz |
2.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...