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Status |
Public on May 14, 2024 |
Title |
Investigation of the Mechanism of Action by Comprehensive Transcriptome Analysis |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To elucidate the SLFN12 RNase effector function, we used whole-genome microarray expression profiling as a discovery platform to identify genes that potentially drive anticancer efficacy upon OPB-171775 treatment. The compound was treated for 8 or 24 hours in control PFSK1 cells and siSLFN12 knockdown PFSK1 cells. Gene set enrichment analysis (GSEA) revealed a significant enrichment of gene sets associated with ribosomal function and the endoplasmic reticulum (ER) stress response in OPB-treated cells compared to control cells.
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Overall design |
PFSK1 cells were treated with siControl and siSLFN12 for 48 hours. Cells were then treated with OPB-171775 or DMSO control for 8 or 24 hours. Three independent experiments were performed at each time point (8 or 24 hours).
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Contributor(s) |
Watanabe T, Kuniyoshi Y, Nakamura K |
Citation(s) |
38864850 |
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Submission date |
Aug 21, 2023 |
Last update date |
Aug 13, 2024 |
Contact name |
Kazuhide Nakamura |
E-mail(s) |
Nakamura.Kazuhide@otsuka.jp
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Organization name |
Otsuka Pharmaceutical Co.,Ltd.
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Department |
Department of Medical Innovations, Osaka Research Center for Drug Discovery
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Lab |
Oncology
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Street address |
5-1-35, Saito-aokita,
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City |
Minoh |
State/province |
Osaka |
ZIP/Postal code |
562-0029 |
Country |
Japan |
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Platforms (1) |
GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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Samples (24)
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Relations |
BioProject |
PRJNA1007669 |