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Series GSE241312 Query DataSets for GSE241312
Status Public on May 14, 2024
Title Investigation of the Mechanism of Action by Comprehensive Transcriptome Analysis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To elucidate the SLFN12 RNase effector function, we used whole-genome microarray expression profiling as a discovery platform to identify genes that potentially drive anticancer efficacy upon OPB-171775 treatment. The compound was treated for 8 or 24 hours in control PFSK1 cells and siSLFN12 knockdown PFSK1 cells. Gene set enrichment analysis (GSEA) revealed a significant enrichment of gene sets associated with ribosomal function and the endoplasmic reticulum (ER) stress response in OPB-treated cells compared to control cells.
 
Overall design PFSK1 cells were treated with siControl and siSLFN12 for 48 hours. Cells were then treated with OPB-171775 or DMSO control for 8 or 24 hours. Three independent experiments were performed at each time point (8 or 24 hours).
 
Contributor(s) Watanabe T, Kuniyoshi Y, Nakamura K
Citation(s) 38864850
Submission date Aug 21, 2023
Last update date Aug 13, 2024
Contact name Kazuhide Nakamura
E-mail(s) Nakamura.Kazuhide@otsuka.jp
Organization name Otsuka Pharmaceutical Co.,Ltd.
Department Department of Medical Innovations, Osaka Research Center for Drug Discovery
Lab Oncology
Street address 5-1-35, Saito-aokita,
City Minoh
State/province Osaka
ZIP/Postal code 562-0029
Country Japan
 
Platforms (1)
GPL21185 Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version]
Samples (24)
GSM7720961 siNTC_DMSO_8h_rep1
GSM7720962 siNTC_OPB_8h_rep1
GSM7720963 siSLFN12_DMSO_8h_rep1
Relations
BioProject PRJNA1007669

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241312_RAW.tar 74.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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