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| Status |
Public on May 14, 2024 |
| Title |
Investigation of the Mechanism of Action by Comprehensive Transcriptome Analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To elucidate the SLFN12 RNase effector function, we used whole-genome microarray expression profiling as a discovery platform to identify genes that potentially drive anticancer efficacy upon OPB-171775 treatment. The compound was treated for 8 or 24 hours in control PFSK1 cells and siSLFN12 knockdown PFSK1 cells. Gene set enrichment analysis (GSEA) revealed a significant enrichment of gene sets associated with ribosomal function and the endoplasmic reticulum (ER) stress response in OPB-treated cells compared to control cells.
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| Overall design |
PFSK1 cells were treated with siControl and siSLFN12 for 48 hours. Cells were then treated with OPB-171775 or DMSO control for 8 or 24 hours. Three independent experiments were performed at each time point (8 or 24 hours).
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| Contributor(s) |
Watanabe T, Kuniyoshi Y, Nakamura K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 21, 2023 |
| Last update date |
May 14, 2024 |
| Contact name |
Kazuhide Nakamura |
| E-mail(s) |
Nakamura.Kazuhide@otsuka.jp
|
| Organization name |
Otsuka Pharmaceutical Co.,Ltd.
|
| Department |
Department of Medical Innovations, Osaka Research Center for Drug Discovery
|
| Lab |
Oncology
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| Street address |
5-1-35, Saito-aokita,
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| City |
Minoh |
| State/province |
Osaka |
| ZIP/Postal code |
562-0029 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA1007669 |
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