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| Status |
Public on Jan 10, 2024 |
| Title |
Expression data from C2C12 myotubes treated with sugarcane top ethanolic extract (STEE) and control C2C12 myotubes. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of the study was to obtain the data on the changes brought by STEE in in vitro myotubes, with a focus on metabolism and mitochondria, to explore its unknown functional properties. In this study, the transcriptome-wide analysis using microarray was performed, whcih provide insights into the biological and molecular changes induced by STEE.
The microarray allowed us to capture the transcriptome-wide changes induced by STEE in C2C12 myotubes. For example, STEE induced changes in the expression of genes involved in mitochondria activity, fatty acid metabolism, or inflammatory cytokines in C2C12 myotubes.
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| Overall design |
Microarray analysis was performed in duplicates for each samples; STEE treatment groups were set at two concentrations, 30 µg/mL and 50 µg/mL, and exposed to C2C12 myotubes for 6 hours. RNA extraction from control C2C12 myotubes followed the same schedule as the extraction from STEE-treated C2C12 myotubes.
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| Contributor(s) |
Iwata K |
| Citation(s) |
38177614 |
| Submission date |
Sep 18, 2023 |
| Last update date |
Jan 11, 2024 |
| Contact name |
Kengo Iwata |
| Organization name |
University of Tsukuba / Nippo., Co., Ltd.
|
| Department |
Alliance for Research on the Mediterranean and North Africa (ARENA)
|
| Street address |
1-1-1 Tennodai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8572 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (6)
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| GSM7785341 |
C2C12 myotubes_30 µg/mL STEE-treated_6 hours_rep2 |
| GSM7785342 |
C2C12 myotubes_50 µg/mL STEE-treated_6 hours_rep1 |
| GSM7785343 |
C2C12 myotubes_50 µg/mL STEE-treated_6 hours_rep2 |
|
| Relations |
| BioProject |
PRJNA1018453 |
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