|
| Status |
Public on Apr 30, 2011 |
| Title |
Systems biology approach to identify transcriptional reprogramming and microRNA targets during the progression of Polycystic Kidney Disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by cyst formation throughout the kidney parenchyma. It is caused by mutations in either of two genes, PKD1 and PKD2. Mice that lack functional Pkd1 (Pkd1null/null), develop rapidly progressive cystic disease during embryogenesis, and serve as a model to study human ADPKD. We examined the molecular pathways that modulate renal cyst growth in the Pkd1null/null model by performing global gene-expression proļ¬ling in embryonic kidneys at day 14 and 17. Gene Ontology and gene set enrichment analysis were used to identify overrepresented signaling pathways in Pkd1null/null kidneys. We found dysregulation of developmental, metabolic, and signaling pathways (e.g. Wnt, calcium, TGF-b and MAPK) in Pkd1null/null kidneys.
|
| |
|
| Overall design |
Total RNA were obtained from kidneys of wild-type and Pkd1null/null animals at embryonic ages 14.5 and 17.5.
|
| |
|
| Contributor(s) |
Pandey P, Kreidberg JA |
| Citation(s) |
21518438 |
| Submission date |
Sep 24, 2010 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Priyanka Pandey |
| E-mail(s) |
Priyanka.Pandey@childrens.harvard.edu
|
| Organization name |
Children's Hospital Boston
|
| Department |
Medicine/Nephrology
|
| Street address |
Children's Hospital Boston, 300 Longwood avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (12)
|
| GSM600319 |
Embryonic age 14.5 wild-type rep1 (PKD1_34) |
| GSM600320 |
Embryonic age 14.5 wild-type rep1 (PKD1_57) |
| GSM600321 |
Embryonic age 14.5 wild-type rep1 (PKD1_58) |
| GSM600322 |
Embryonic age 14.5 mutant rep2 (PKD1_35) |
| GSM600323 |
Embryonic age 14.5 mutant rep2 (PKD1_59) |
| GSM600324 |
Embryonic age 14.5 mutant rep2 (PKD1_65) |
| GSM600325 |
Embryonic age 17.5 wild-type rep3 (PKD1_2) |
| GSM600326 |
Embryonic age 17.5 wild-type rep3 (PKD1_38) |
| GSM600327 |
Embryonic age 17.5 wild-type rep3 (PKD1_41) |
| GSM600328 |
Embryonic age 17.5 mutant rep4 (PKD1_12) |
| GSM600329 |
Embryonic age 17.5 mutant rep4 (PKD1_40) |
| GSM600330 |
Embryonic age 17.5 mutant rep4 (PKD1_42) |
|
| Relations |
| BioProject |
PRJNA132803 |
Less...
More...