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| Status |
Public on May 17, 2011 |
| Title |
The transcriptome of human epicardial, mediastinal and subcutaneous adipose tissues in men with coronary artery disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The biological functions of epicardial adipose tissue (EAT) remain largely elusive. However, the proximity of EAT to the coronary arteries suggests a role in the pathogenesis of coronary artery disease (CAD). Objectives of this study were to identify genes that are up- or down-regulated among three adipose tissues (AT), namely EAT, mediastinal (MAT) and subcutaneous (SAT) and to study their possible relationships with the development of cardiovascular diseases. Samples were collected from patients undergoing coronary artery bypass grafting surgeries. Gene expression was evaluated in all three AT depots of six men using the lllumina® HumanWG-6 v3.0 expression BeadChips. Twenty-two and 73 genes were up-regulated in EAT compared to mediastinal and subcutaneous AT, respectively. Ninety-four genes were down-regulated in EAT compared to subcutaneous adipose depot. However, none were significantly down-regulated in EAT compared to mediastinal fat. More specifically, the expression of the adenosine A1 receptor (ADORA1), involved in myocardial ischemia, was significantly up-regulated in EAT. Levels of the prostaglandin D2 synthase (PTGDS) gene, recently associated with the progression of atherosclerosis, were significantly different in the three pairwise comparisons (epicardial > mediastinal > subcutaneous). The results of ADORA1 and PTGDS were confirmed by qPCR in 25 biological replicates. Overall, the transcriptional profiles of EAT and MAT were similar compared to the subcutaneous compartment. Despite this similarity, two genes involved in cardiovascular diseases, ADORA1 and PTGDS, were up-regulated in EAT. These results provide insights about the biology of EAT and its potential implication in CAD.
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| Overall design |
Samples of epicardial, mediastinal and subscutaneous adipose tissue were collected from the chest of 6 male patients undergoing coronary artery bypass grafting surgeries. The RNA was labeled and hybridized using a standard Illumina protocol (https://icom.illumina.com/Login?ReturnUrl=%2ficom%2fsoftware.ilmn%3fid%3d214&id=214). Three pairwise comparison among the three adipose tissue were made using significance analysis of microarrays.
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| Contributor(s) |
Guauque-Olarte S, Mathieu P, Bossé Y |
| Citation(s) |
21603615 |
| Submission date |
Sep 29, 2010 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Sandra Guauque-Olarte |
| E-mail(s) |
sandra-milena.guauque.1@ulaval.ca
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| Phone |
418-6568711
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| Organization name |
CRIUCPQ
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| Street address |
2725, chemin Sainte-Foy
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| City |
Quebec |
| ZIP/Postal code |
G1V 4G5 |
| Country |
Canada |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA132613 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24425_IDAT.tar.gz |
2.6 Gb |
(ftp)(http) |
TAR |
| GSE24425_RAW.tar |
6.3 Mb |
(http)(custom) |
TAR |
| GSE24425_non-normalized.txt.gz |
5.7 Mb |
(ftp)(http) |
TXT |
| GSE24425_normalized.txt.gz |
6.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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