 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 06, 2010 |
| Title |
Expression data of influenza A-infected human type I-like alveolar epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Pandemic influenza H1N1 (pdmH1N1) virus causes mild disease in humans but occasionally leads to severe complications and even death, especially in those who are pregnant or have underlying disease. Cytokine responses induced by pdmH1N1 viruses in vitro are comparable to other seasonal influenza viruses, suggesting the cytokine dysregulation as seen in H5N1 infection is not a feature of the pdmH1N1 virus. However, a comprehensive gene expression profile of pdmH1N1 in relevant primary human cells in vitro has not been reported. Type I alveolar epithelial cells are a key target cell in pdmH1N1 pneumonia. We carried out a comprehensive gene expression profiling using the Affymetrix microarray platform to compare the transcriptomes of primary human alveolar type I-like alveolar epithelial cells infected with pdmH1N1 or seasonal H1N1 virus.
|
| |
|
| Overall design |
Primary type II alveolar epithelial cells were isolated from human non-malignant lung tissue of three patients who underwent lung resection, and cells were differentiated to type I-like before use. Type I-like alveolar epithelial cells were mock infected, or infected with pdmH1N1 or seasonal H1N1 viruses at a multiplicity of infection (MOI) of two. Total RNA was extracted from cells after 8h post-infection, and gene expression profiling was performed using an Affymetrix Human Gene 1.0 ST microarray platform.
|
| |
|
| Contributor(s) |
Lee SM, Chan RW, Gardy JL, Lo CK, Sihoe AD, Kang SS, Cheung TK, Guan Y, Chan MC, Hancock RE, Peiris JM |
| Citation(s) |
21029402 |
| Submission date |
Oct 05, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
SMY Lee |
| Organization name |
The University of Hong Kong
|
| Street address |
21 Sassoon Road
|
| City |
Hong Kong |
| ZIP/Postal code |
HKG |
| Country |
Hong Kong |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (9)
|
| GSM604841 |
Donor 1, mock-infected human type I-like alveolar epithelial cells at 8h |
| GSM604842 |
Donor 1, seasonal H1N1-infected human type I-like alveolar epithelial cells at 8h |
| GSM604843 |
Donor 1, pdmH1N1-infected human type I-like alveolar epithelial cells at 8h |
| GSM604844 |
Donor 2, mock-infected human type I-like alveolar epithelial cells at 8h |
| GSM604845 |
Donor 2, seasonal H1N1-infected human type I-like alveolar epithelial cells at 8h |
| GSM604846 |
Donor 2, pdmH1N1-infected human type I-like alveolar epithelial cells at 8h |
| GSM604847 |
Donor 3, mock-infected human type I-like alveolar epithelial cells at 8h |
| GSM604848 |
Donor 3, seasonal H1N1-infected human type I-like alveolar epithelial cells at 8h |
| GSM604849 |
Donor 3, pdmH1N1-infected human type I-like alveolar epithelial cells at 8h |
|
| Relations |
| BioProject |
PRJNA132555 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24533_RAW.tar |
35.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...