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| Status |
Public on Mar 07, 2011 |
| Title |
Signal-dependent incorporation of MyoD-BAF60c into Brg1-based SWI/SNF chromatin-remodeling complex |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Tissue-specific transcription factors initiate differentiation toward a specialized cell type by inducing transcription-permissive chromatin modifications at target gene promoters, through the recruitment of the SWI/SNF chromatin-remodeling complex (1, 2). The molecular mechanism that regulates the chromatin re-distribution of SWI/SNF in response to differentiation signals is currently unknown. Here we show that the muscle determination factor MyoD and the SWI/SNF structural sub-unit, BAF60c (SMARCD3), form a complex on the regulatory elements of MyoD-target genes in undifferentiated myoblasts, prior to the activation of gene expression. MyoD-BAF60c complex is devoid of the ATP-dependent enzymatic sub-units Brg1 and Brm, is required for stable MyoD binding to Ebox sequences, and marks the chromatin for signal-dependent recruitment of the SWI/SNF core complex to muscle loci. BAF60c phosphorylation on a conserved threonine by differentiation-activated p38 signalling promotes the incorporation of MyoD-BAF60c into a Brg1-based SWI/SNF complex, which is competent to remodel the chromatin and activates transcription of MyoD-target genes. Our data support an unprecedented two-step model, by which pre-assembled BAF60c-MyoD complex directs the SWI/SNF complex chromatin re-distribution to muscle loci in response to differentiation cues.
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| Overall design |
Differentiation of C2C12 cells individually interfered for BRG1, BAF60B, BAF60C
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| Citation(s) |
22068056 |
| Submission date |
Oct 07, 2010 |
| Last update date |
Sep 24, 2019 |
| Contact name |
Roy M Williams |
| E-mail(s) |
rwillia@scripps.edu
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| Organization name |
The Scripps Research Institute
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| Department |
Regenerative Medicine
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| Lab |
Loring
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| Street address |
3030 Science Park Road
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| GSM605690 |
gene expression of wild type C2C12 cells in growth media replicate 1 |
| GSM605691 |
gene expression of wild type C2C12 cells in differentiation media replicate 1 |
| GSM605692 |
gene expression of C2C12-siScramble cells in differentiation media replicate 1 |
| GSM605693 |
gene expression of C2C12 siBRG1 cells in differentiation media replicate 1 |
| GSM605694 |
gene expression of C2C12 siBAF60b cells, in differentiation media replicate 1 |
| GSM605695 |
gene expression of C2C12 siBAF60c cells, in differentiation media replicate 1 |
| GSM605696 |
gene expression of wild type C2C12 cells in growth media replicate 2 |
| GSM605697 |
gene expression of wild type C2C12 cells in differentiation media replicate 2 |
| GSM605698 |
gene expression of C2C12-siScramble cells in differentiation media replicate 2 |
| GSM605699 |
gene expression of C2C12 siBRG1 cells in differentiation media replicate 2 |
| GSM605700 |
gene expression of C2C12 siBAF60b cells, in differentiation media replicate 2 |
| GSM605701 |
gene expression of C2C12 siBAF60c cells, in differentiation media replicate 2 |
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| Relations |
| BioProject |
PRJNA132415 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24573_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE24573_non-normalized_data.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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