|
| Status |
Public on Jan 18, 2012 |
| Title |
Potentiation of glucocorticoid signaling by chloroquine via inhibition of lysosomes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Anti-malaria drug chloroquine has been used as an anti-inflammatory agent for treating systemic lupus erythematosus and rheumatoid arthritis, without clear mechanism of action. Here we report that chloroquine potently inhibits the expression of proinflammatory cytokines through transrepression of glucocorticoid receptor (GR). Instead of direct binding to GR, chloroquine synergistically activates glucocorticoid signaling via inhibition of lysosomal functions. In mouse collagen induced arthritis model, chloroquine synergizes the therapeutic effects of glucocorticoid. Lysosomal inhibition by bafilomycin A1, an inhibitor of V-type ATPase, or by knockdown of transcription factor EB (TFEB), a master activator of lysosomal biogenesis, mimics the effects of chloroquine. These results reveal an unexpected regulation of glucocorticoid signaling by lysosomes and provide a mechanistic basis for treating inflammation and autoimmune diseases by combination of glucocorticoids and lysosomal inhibitors.
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| |
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| Overall design |
Macrophage THP-1 cells treated with LPS, chloroquine and dexamethasone.
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| |
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| Contributor(s) |
Dykema K |
| Citation(s) |
21730326 |
| Submission date |
Oct 07, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Karl Dykema |
| Organization name |
Van Andel Institute
|
| Lab |
Lab of Computational Biology
|
| Street address |
333 Bostwick Ave. NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (4)
|
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| Relations |
| BioProject |
PRJNA132479 |
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