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| Status |
Public on Mar 31, 2011 |
| Title |
Epstein–Barr virus (EBV) Rta-mediated cell cycle arrest enables permissive replication of EBV and Kaposi’s sarcoma-associated herpesvirus in 293 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Epstein-Barr virus (EBV) Rta is a latent-lytic molecular switch evolutionarily conserved in all gamma-herpesviruses. In previous studies, doxycycline-inducible Rta was shown to potently produce an irreversible G1 arrest followed by cellular senescence in 293 cells. Here, we demonstrate that in this system the inducible Rta not only reactivates resident genome of EBV but also that of Kaposi’s sarcoma-associated herpesvirus (KSHV), to similar efficiency. However, Rta-induced senescence program was terminated by the robust viral lytic cycle replication that eventually caused cell death. Furthermore, prior to the abrupt expression of immediate-early protein (EBV BZLF1 or KSHV RTA), Rta simultaneously down-regulates cell cycle activators (c-Myc, CDK6, CCND2) and up-regulates senescence-related genes (p21, 14-3-3s). Since Rta is a viral immediate-early transcriptional activator, it is envisioned that during the initial stage of viral reactivation, Rta may engage to modulate the host transcriptome, to halt cell cycle progression, and to maintain an ideal environment for manufacturing infectious virions.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
Refer to individual Series.
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| Contributor(s) |
Lin S, Chen Y, Tsai W, Chen Y, Ko Y |
| Citation(s) |
21423768, 21918011 |
| Submission date |
Oct 07, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Su-Fang Lin |
| E-mail(s) |
sflin1@gmail.com
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| Organization name |
National Health Research Institutes, Taiwan
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| Department |
NICR
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| Lab |
R2-1211
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| Street address |
No 35, Keyan Road, Zhunan Town
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| City |
Miaoli Conunty |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (7)
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| GSM606063 |
Control, 293TetLuc, doxcycline 48 h, rep1 |
| GSM606064 |
Control, 293TetLuc, doxcycline 48 h, rep2 |
| GSM606065 |
293TetER doxycycline 48 h, rep1 |
| GSM606066 |
293TetER doxycycline 48 h, rep2 |
| GSM606067 |
Control, doxcycline 24 h, rep 1 |
| GSM606068 |
TW01TetER_cl7, doxycycline 24 h, rep1 |
| GSM606069 |
TW01TetER_cl19, doxycycline 24 h, rep1 |
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| This SuperSeries is composed of the following SubSeries: |
| GSE24585 |
Expression profiling of host genes modulated by Epstein-Barr virus (EBV) Rta in HEK293 cells |
| GSE24586 |
Expression profiling of host genes modulated by Epstein-Barr virus Rta in nasopharyngeal carcinoma cells |
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| Relations |
| BioProject |
PRJNA132491 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24587_RAW.tar |
30.1 Mb |
(http)(custom) |
TAR (of CEL) |
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