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Series GSE246339

Query DataSets for GSE246339

Status

Public on Oct 31, 2023

Title

Expression data from macrophages sorted from the heart tissue of WT mice post-IR

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Cardiac resident MerTK+ macrophages exert multiple protective roles post-ischemic injury. To determine the potential reason for the protective role of MerTK+ cardiac macrophages, microarray was performed to identify gene expression profiles on isolated Trem2+, MHCII+, Lyve1+, and MerTK- macrophages from the heart tissue of WT mice post-IR.
We investigated the potential reason for the protective role of MerTK+ cardiac macrophages in myocardial Ischemia reperfusion injuryby microarray.

Overall design

Trem2+, MHCII+, Lyve1+, and MerTK- macrophages were isolated from the heart tissue of WT mice post-IR. Total RNA was isolated with TRIzol (Invitrogen) from macrophages according to manufacturer’s instructions. Gene expression profiling was performed using Affymetrix GeneChip mouse Genome 430 2.0 array according to the manufacturer’s instructions (Affymetrix, Inc., Santa Clara, CA). On the GeneChip Mouse Genome 430 2.0 Array, over 45,000 probe sets analyze the expression level of over 39,000 transcripts and variants from over 34,000 well characterized mouse genes.

Contributor(s)

Li Y, Shao Y

Citation missing

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Submission date

Oct 26, 2023

Last update date

Nov 01, 2023

Contact name

李玉琳

Organization name

Beijing Institute of Heart Lung and Blood Vessel Diseases