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| Status |
Public on Jun 01, 2011 |
| Title |
Genome-wide analysis of GATA1, GATA2, RUNX1, FLI1 and SCL binding in primary human megakaryocytes |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We have mapped the binding sites for the five key regulators GATA1, GATA2, RUNX1, FLI1 and TAL1/SCL in primary human megakaryocytes. Statistical analysis identified subsets of enriched as well as depleted combinatorial binding patterns. In particular simultaneous binding by all 5 factors was highly enriched and occurred in the vicinity of many genes known to be involved in blood and megakaryocyte development. Knock down in zebrafish of 8 of these genes with no previously known role in hematopoiesis, revealed all to be essential for thrombocyte and/or erythroid development. Combinatorial analysis of multi-factor ChIP-Seq datasets coupled with a high-throughput in vivo screen therefore offers a powerful strategy to identify novel essential regulators of complex mammalian differentiation processes.
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| |
|
| Overall design |
5 transcription factors and rabbit-IgG in megakaryocytes.
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| |
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| Contributor(s) |
Tijssen MR, Cvejic A, Hannah RL, Joshi A, Wang X, Bellissimo DC, Ouwehand WH, Gottgens B |
| Citation(s) |
21571218 |
| Submission date |
Oct 13, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Samuel David Foster |
| E-mail(s) |
sdf21@cam.ac.uk
|
| Organization name |
Cambridge Institute for Medical Research
|
| Department |
Department of Haematology
|
| Lab |
Haematopoietic Stem Cell Lab
|
| Street address |
Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| SRA |
SRP003927 |
| BioProject |
PRJNA132361 |
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