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Status |
Public on May 14, 2024 |
Title |
Non-classical CD45RBlo memory B-cells are the majority of circulating antigen-specific B-cells following mRNA vaccination and COVID-19 infection. |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Resting memory B-cells can be divided into classical and non-classical groups based on differential expression of markers such as CD27 and CD11c, while activated memory B-cells express a combination of markers, making their ontogeny hard to determine. Here by longitudinal analysis of COVID-19, bacterial sepsis, and BNT162b2 mRNA vaccine recipients by mass cytometry and CITE-seq we describe a three-branch structure of resting B-cell memory consisting of “classical” CD45RB⁺ memory and two branches of CD45RB^lo memory further defined by expression of CD23 and CD11c respectively. Stable differences in CD45RB upon activation allowed tracking of activated B-cells and plasmablasts derived from CD45RB⁺ classical and CD45RB^lo non-classical memory B-cells. In both COVID-19 patients and mRNA vaccination, CD45RB^lo B-cells formed the majority of SARS-CoV2 specific memory B-cells while CD45RB⁺ memory was most strongly activated by bacterial Sepsis. These results suggest that diverse non-classical CD45RB^lo memory B-cells consisting of branches of CD11c⁺Tbet⁺ and CD23⁺ fractions form a critical part of responses to viral infection and vaccination.
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Overall design |
Frozen human PBMCs from COVID-19 patients, Sepsis patients or mRNA vaccinated individuals were defrosted then hashtagged with TotalSeqC hashtags. Hashtagged samples were then mixed and stained with Flourecently labelled antibodies, surface TotalSeqC antibodies and SARS-CoV2 spike protein-Strepaaviding tetramers. IgD negative B-cells were FACS sorted as IgD-CD19+CD14-CD4-.
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Contributor(s) |
Wing JB |
Citation missing |
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Submission date |
Nov 10, 2023 |
Last update date |
May 14, 2024 |
Contact name |
Daisuke Motooka |
Organization name |
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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Street address |
3-1, Yamadaoka
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City |
Suita |
State/province |
Osaka |
ZIP/Postal code |
5650871 |
Country |
Japan |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (15)
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Relations |
BioProject |
PRJNA1038765 |
Supplementary file |
Size |
Download |
File type/resource |
GSE247488_ADT_HTO_details_README.txt |
2.0 Kb |
(ftp)(http) |
TXT |
GSE247488_B-cells_1_airr_rearrangement.tsv.gz |
3.1 Mb |
(ftp)(http) |
TSV |
GSE247488_B-cells_1_clonotypes.csv.gz |
146.0 Kb |
(ftp)(http) |
CSV |
GSE247488_B-cells_2_airr_rearrangement.tsv.gz |
4.4 Mb |
(ftp)(http) |
TSV |
GSE247488_B-cells_2_clonotypes.csv.gz |
196.1 Kb |
(ftp)(http) |
CSV |
GSE247488_B-cells_3_airr_rearrangement.tsv.gz |
3.5 Mb |
(ftp)(http) |
TSV |
GSE247488_B-cells_3_clonotypes.csv.gz |
150.7 Kb |
(ftp)(http) |
CSV |
GSE247488_B-cells_4_airr_rearrangement.tsv.gz |
3.6 Mb |
(ftp)(http) |
TSV |
GSE247488_B-cells_4_clonotypes.csv.gz |
155.1 Kb |
(ftp)(http) |
CSV |
GSE247488_B-cells_5_airr_rearrangement.tsv.gz |
3.5 Mb |
(ftp)(http) |
TSV |
GSE247488_B-cells_5_clonotypes.csv.gz |
161.0 Kb |
(ftp)(http) |
CSV |
GSE247488_features.tsv.gz |
325.8 Kb |
(ftp)(http) |
TSV |
GSE247488_matrix_B-cells_1.csv.gz |
20.9 Mb |
(ftp)(http) |
CSV |
GSE247488_matrix_B-cells_2.csv.gz |
27.9 Mb |
(ftp)(http) |
CSV |
GSE247488_matrix_B-cells_3.csv.gz |
30.7 Mb |
(ftp)(http) |
CSV |
GSE247488_matrix_B-cells_4.csv.gz |
28.6 Mb |
(ftp)(http) |
CSV |
GSE247488_matrix_B-cells_5.csv.gz |
26.2 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
Raw data are available in SRA |