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Series GSE247488 Query DataSets for GSE247488
Status Public on May 14, 2024
Title Non-classical CD45RBlo memory B-cells are the majority of circulating antigen-specific B-cells following mRNA vaccination and COVID-19 infection.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Resting memory B-cells can be divided into classical and non-classical groups based on differential expression of markers such as CD27 and CD11c, while activated memory B-cells express a combination of markers, making their ontogeny hard to determine. Here by longitudinal analysis of COVID-19, bacterial sepsis, and BNT162b2 mRNA vaccine recipients by mass cytometry and CITE-seq we describe a three-branch structure of resting B-cell memory consisting of “classical” CD45RB⁺ memory and two branches of CD45RB^lo memory further defined by expression of CD23 and CD11c respectively. Stable differences in CD45RB upon activation allowed tracking of activated B-cells and plasmablasts derived from CD45RB⁺ classical and CD45RB^lo non-classical memory B-cells. In both COVID-19 patients and mRNA vaccination, CD45RB^lo B-cells formed the majority of SARS-CoV2 specific memory B-cells while CD45RB⁺ memory was most strongly activated by bacterial Sepsis. These results suggest that diverse non-classical CD45RB^lo memory B-cells consisting of branches of CD11c⁺Tbet⁺ and CD23⁺ fractions form a critical part of responses to viral infection and vaccination.
 
Overall design Frozen human PBMCs from COVID-19 patients, Sepsis patients or mRNA vaccinated individuals were defrosted then hashtagged with TotalSeqC hashtags. Hashtagged samples were then mixed and stained with Flourecently labelled antibodies, surface TotalSeqC antibodies and SARS-CoV2 spike protein-Strepaaviding tetramers. IgD negative B-cells were FACS sorted as IgD-CD19+CD14-CD4-.
 
Contributor(s) Wing JB
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 10, 2023
Last update date May 14, 2024
Contact name Daisuke Motooka
Organization name Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
Street address 3-1, Yamadaoka
City Suita
State/province Osaka
ZIP/Postal code 5650871
Country Japan
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (15)
GSM7890660 B-cells_1_BCR
GSM7890661 B-cells_1_CITE
GSM7890662 B-cells_1_GEX
Relations
BioProject PRJNA1038765

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE247488_ADT_HTO_details_README.txt 2.0 Kb (ftp)(http) TXT
GSE247488_B-cells_1_airr_rearrangement.tsv.gz 3.1 Mb (ftp)(http) TSV
GSE247488_B-cells_1_clonotypes.csv.gz 146.0 Kb (ftp)(http) CSV
GSE247488_B-cells_2_airr_rearrangement.tsv.gz 4.4 Mb (ftp)(http) TSV
GSE247488_B-cells_2_clonotypes.csv.gz 196.1 Kb (ftp)(http) CSV
GSE247488_B-cells_3_airr_rearrangement.tsv.gz 3.5 Mb (ftp)(http) TSV
GSE247488_B-cells_3_clonotypes.csv.gz 150.7 Kb (ftp)(http) CSV
GSE247488_B-cells_4_airr_rearrangement.tsv.gz 3.6 Mb (ftp)(http) TSV
GSE247488_B-cells_4_clonotypes.csv.gz 155.1 Kb (ftp)(http) CSV
GSE247488_B-cells_5_airr_rearrangement.tsv.gz 3.5 Mb (ftp)(http) TSV
GSE247488_B-cells_5_clonotypes.csv.gz 161.0 Kb (ftp)(http) CSV
GSE247488_features.tsv.gz 325.8 Kb (ftp)(http) TSV
GSE247488_matrix_B-cells_1.csv.gz 20.9 Mb (ftp)(http) CSV
GSE247488_matrix_B-cells_2.csv.gz 27.9 Mb (ftp)(http) CSV
GSE247488_matrix_B-cells_3.csv.gz 30.7 Mb (ftp)(http) CSV
GSE247488_matrix_B-cells_4.csv.gz 28.6 Mb (ftp)(http) CSV
GSE247488_matrix_B-cells_5.csv.gz 26.2 Mb (ftp)(http) CSV
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