 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 01, 2011 |
| Title |
Expression profile of human natural killer cells co-cultured with P. falciparum infected erythrocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to identify an expression signature that might characterize the impact of Plasmodium parasites on NK cells, we have used Affymetrix oligonucleotide microarrays to examin the global gene expression profile of primary NK cells that have been co-incubated with P. falciparum infected red blood cells and have compared it to the expression pattern of NK genes induced by cytokine treatment.
|
| |
|
| Overall design |
PBMCs were isolated from 3 healthy donors and were incubated with iRBCs, uRBCs, IL12 plus IL18 and without any stimulus. After 24 hours at 37ºC and 5%CO2, Natural killer cells were isolated by negative selection, checked for purity by flow cytometry and processed for RNA extraction and analysis with the Human Gene 1.0 Affymetrix array. The experiment was repeated 3 times (1-2 weeks apart) for each one of the 3 donors.
|
| |
|
| Contributor(s) |
de Carvalho EG, Bonin M, Kun JF |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 19, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Michael Bonin |
| Organization name |
University Tuebingen
|
| Department |
Medical Genetics
|
| Lab |
Microarray Facility
|
| Street address |
CAlwerstr. 7
|
| City |
Tuebingen |
| State/province |
BW |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (30)
|
| GSM610486 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor1, iRBCs, replicate1] |
| GSM610487 |
NK cells whithin PBMCs that were co-incubated with uninfected red blood cells [Donor1, uRBCs, replicate1] |
| GSM610488 |
NK cells whithin PBMCs that were co-incubated with a mix of IL12 plus IL18 [Donor1, ILmix, replicate1] |
| GSM610489 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor1, untreated, replicate1] |
| GSM610490 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor1, iRBCs, replicate2] |
| GSM610491 |
NK cells whithin PBMCs that were co-incubated with uninfected red blood cells [Donor1, uRBCs, replicate2] |
| GSM610492 |
NK cells whithin PBMCs that were co-incubated with a mix of IL12 plus IL18 [Donor1, ILmix, replicate2] |
| GSM610493 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor1, untreated, replicate2] |
| GSM610494 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor1, iRBCs, replicate3] |
| GSM610495 |
NK cells whithin PBMCs that were co-incubated with uninfected red blood cells [Donor1, uRBCs, replicate3] |
| GSM610496 |
NK cells whithin PBMCs that were co-incubated with a mix of IL12 plus IL18 [Donor1, ILmix, replicate3] |
| GSM610497 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor1, untreated, replicate3] |
| GSM610498 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor2, iRBCs, replicate1] |
| GSM610499 |
NK cells whithin PBMCs that were co-incubated with uninfected red blood cells [Donor2, uRBCs, replicate1] |
| GSM610500 |
NK cells whithin PBMCs that were co-incubated with a mix of IL12 plus IL18 [Donor2, ILmix, replicate1] |
| GSM610501 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor2, untreated, replicate1] |
| GSM610502 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor2, iRBCs, replicate2] |
| GSM610503 |
NK cells whithin PBMCs that were co-incubated with uninfected red blood cells [Donor2, uRBCs, replicate2] |
| GSM610504 |
NK cells whithin PBMCs that were co-incubated with a mix of IL12 plus IL18 [Donor2, ILmix, replicate2] |
| GSM610505 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor2, untreated, replicate2] |
| GSM610506 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor2, iRBCs, replicate3] |
| GSM610507 |
NK cells whithin PBMCs that were co-incubated with uninfected red blood cells [Donor2, uRBCs, replicate3] |
| GSM610508 |
NK cells whithin PBMCs that were co-incubated with a mix of IL12 plus IL18 [Donor2, ILmix, replicate3] |
| GSM610509 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor2, untreated, replicate3] |
| GSM610510 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor3, iRBCs, replicate1] |
| GSM610511 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor3, untreated, replicate1] |
| GSM610512 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor3, iRBCs, replicate2] |
| GSM610513 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor3, untreated, replicate2] |
| GSM610514 |
NK cells whithin PBMCs that were co-incubated with P. falciparum infected red blood cells [Donor3, iRBCs, replicate3] |
| GSM610515 |
NK cells whithin PBMCs that were incubated in culture medium only (no stimulus) [Donor3, untreated, replicate3] |
|
| Relations |
| BioProject |
PRJNA132247 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24791_RAW.tar |
124.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...