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| Status |
Public on May 09, 2024 |
| Title |
Identification of differentially expressed tumour-related genes regulated by UHRF1 regulated DNA methylation [WGBS] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
UHRF1 is an epigenetic regulator that plays critical roles in tumours. However, the DNA methylation alteration patterns driven by UHRF1 and the related differentially expressed tumour-related genes remain unclear. In this study, a UHRF1-shRNA MCF-7 cell line was constructed, and whole-genome bisulfite sequencing (WGBS) and RNA sequencing were performed. The DNA methylation alteration landscape was elucidated, and DNA methylation-altered regions (DMRs) were found to be distributed in both gene bodies and adjacent regions. The DMRs were annotated and categorized into 488 hypermethylated/1696 hypomethylated promoters and 1149 hypermethylated/5501 hypomethylated gene bodies. Through an integrated analysis with the RNA sequencing data, 217 upregulated methylated genes and 288 downregulated methylated genes were identified, and these genes were primarily enriched in nervous system development and cancer signalling pathways.
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| Overall design |
UHRF1-lentivirusVS scramble-lentivirus
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| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 26, 2023 |
| Last update date |
May 09, 2024 |
| Contact name |
Gong chun xue |
| E-mail(s) |
q21201060@stu.ahu.edu.cn
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| Organization name |
Beijing Institute of Biotechnology
|
| Street address |
20 East Street, Fengtai District
|
| City |
Beijing |
| ZIP/Postal code |
100850 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE248622 |
Identification of differentially expressed tumour-related genes regulated by UHRF1 regulated DNA methylation |
|
| Relations |
| BioProject |
PRJNA1045262 |
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