|
| Status |
Public on Dec 10, 2010 |
| Title |
Splenic CD4 T cells in naïve C57BL/6 mice or during Plasmodium berghei ANKA infection in C57BL/6 and B6.GzmB-/- mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Infection of C57BL/6 mice with Plasmodium berghei ANKA (PbA) is a well-established experimental model of cerebral malaria (ECM). ECM is characterised by high levels of parasite sequestration and recruitment of pathogenic CD8+ T cells to the brain. The role of CD4+ T cells in this model has not yet been fully elucidated, although our laboratory has recently shown that CD4+ T cell depletion prior to infection results in significantly lower parasite burdens and protection from ECM. These data highlight a pathogenic role for CD4+ T cells in ECM.
Our unpublished observations indicate that mice lacking the CD8+ T cell cytolytic effector molecule, Granzyme B, (GzmB), are resistant to ECM, showing markedly reduced parasite burdens. Late depletion of CD4+ T cells from PbA-infected GzmB-deficient mice results in enhanced parasite burdens, indicating that these cells may play an anti-parasitic role. In the present study, we have analysed splenic CD4+ T cell gene expression profiles in naïve C57BL/6 mice, and PbA-infected wild-type and GzmB-deficent mice to further our understanding of the CD4+ T cell response in ECM.
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| Overall design |
Splenic CD4 T cells isolated by cell sorting from either uninfected C57BL/6 or PbA-infected C57BL/6 or B6.GzmB-/- mice at day 4 post-infection. Total RNA obtained from four mice per group.
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| Contributor(s) |
Desbarrieres L, Best SE, Boyle GM, Haque A, Engwerda CR |
| Citation(s) |
21674481, 21525386 |
| Submission date |
Oct 25, 2010 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Ashraful Haque |
| E-mail(s) |
ashraful.haque@qimr.edu.au
|
| Phone |
+61 7 33620414
|
| Fax |
+61 7 38453507
|
| Organization name |
Queensland Institute of Medical Research
|
| Department |
Immunology
|
| Lab |
Immunology and Infection
|
| Street address |
300 Herston Road
|
| City |
Herston |
| State/province |
Brisbane |
| ZIP/Postal code |
QLD 4006 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (12)
|
| GSM612349 |
CD4Tcells_GzmB-/-_PbA-infected_rep1 |
| GSM612350 |
CD4Tcells_C57BL/6_PbA-infected_rep1 |
| GSM612351 |
CD4Tcells_GzmB-/-_PbA-infected_rep2 |
| GSM612352 |
CD4Tcells_C57BL/6_uninfected_rep1 |
| GSM612353 |
CD4Tcells_C57BL/6_PbA-infected_rep2 |
| GSM612354 |
CD4Tcells_C57BL/6_PbA-infected_rep3 |
| GSM612355 |
CD4Tcells_C57BL/6_uninfected_rep2 |
| GSM612356 |
CD4Tcells_GzmB-/-_PbA-infected_rep3 |
| GSM612357 |
CD4Tcells_C57BL/6_uninfected_rep3 |
| GSM612358 |
CD4Tcells_C57BL/6_PbA-infected_rep4 |
| GSM612359 |
CD4Tcells_GzmB-/-_PbA-infected_rep4 |
| GSM612360 |
CD4Tcells_C57BL/6_uninfected_rep4 |
|
| Relations |
| BioProject |
PRJNA132091 |
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